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Items: 1 to 20 of 240

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[Expression, purification and refolding of streptavidin-tagged human tumor necrosis factor-alpha fusion protein].

Xu CX, Hu ZM, Li JL, Gao JM.

Nan Fang Yi Ke Da Xue Xue Bao. 2009 Mar;29(3):412-5. Chinese.

4.

Purification and characterization of RGD tumor-homing peptide conjugated human tumor necrosis factor alpha over-expressed in Escherichia coli.

Ma D, Chen Y, Fang L, Jin G, Zhou B, Cao L, Ye J, Hua Z.

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Oct 1;857(2):231-9. Epub 2007 Jul 22.

PMID:
17716959
5.

Recombinant production of bioactive human TNF-alpha by SUMO-fusion system--high yields from shake-flask culture.

Hoffmann A, Müller MQ, Gloser M, Sinz A, Rudolph R, Pfeifer S.

Protein Expr Purif. 2010 Aug;72(2):238-43. doi: 10.1016/j.pep.2010.03.022. Epub 2010 Apr 2.

PMID:
20363332
6.

Generation of monoclonal antibodies for the assessment of protein purification by recombinant ribosomal coupling.

Kristensen J, Sperling-Petersen HU, Mortensen KK, Sørensen HP.

Int J Biol Macromol. 2005 Dec 15;37(4):212-7. Epub 2005 Dec 2.

PMID:
16330094
7.

An evaluation of different enzymatic cleavage methods for recombinant fusion proteins, applied on des(1-3)insulin-like growth factor I.

Forsberg G, Baastrup B, Rondahl H, Holmgren E, Pohl G, Hartmanis M, Lake M.

J Protein Chem. 1992 Apr;11(2):201-11.

PMID:
1388667
8.

Large-scale immunoaffinity purification of recombinant soluble human antigen CD4 from Escherichia coli cells.

Wells PA, Beiderman B, Garlick RL, Lyle SB, Martin JP Jr, Herberg JT, Meyer HF, Henderson SL, Eckenrode FM.

Biotechnol Appl Biochem. 1993 Dec;18 ( Pt 3):341-57.

PMID:
8297511
9.

Expression in Escherichia coli of the death domain of the human p55 tumor necrosis factor receptor.

De Wilde G, Mertens N, Boone E, De Vreese B, Van Beeumen J, Fiers W, Haegeman G.

Protein Expr Purif. 2001 Nov;23(2):226-32.

PMID:
11676596
10.

High-level production of human growth hormone in Escherichia coli by a simple recombinant process.

Shin NK, Kim DY, Shin CS, Hong MS, Lee J, Shin HC.

J Biotechnol. 1998 Jun 30;62(2):143-51.

PMID:
9706704
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12.

A novel recombinant system for functional expression of myonecrotic snake phospholipase A(2) in Escherichia coli using a new fusion affinity tag.

Seto M, Ogawa T, Kodama K, Muramoto K, Kanayama Y, Sakai Y, Chijiwa T, Ohno M.

Protein Expr Purif. 2008 Apr;58(2):194-202. doi: 10.1016/j.pep.2007.11.013. Epub 2007 Dec 8.

PMID:
18207418
13.

Expression and purification of soluble B lymphocyte stimulator from recombinant Escherichia coli.

Guo QR, Tong WY, Wei DZ, Tao XY.

Prikl Biokhim Mikrobiol. 2008 Mar-Apr;44(2):187-92.

PMID:
18669261
14.

High-level production of a candidacidal peptide lactoferrampin in Escherichia coli by fusion expression.

Yanga Y, Tiana Z, Teng D, Zhang J, Wang J, Wang J.

J Biotechnol. 2009 Feb 23;139(4):326-31.

PMID:
19297728
15.

Expression, purification, and characterization of recombinant protein GX1-rmhTNFalpha.

Cao S, Liu Y, Li X, Zhang Y, Wang J, Du W, Han Y, Jin H, Zhao L, Wu K, Fan D.

Mol Biotechnol. 2009 Sep;43(1):1-7. doi: 10.1007/s12033-009-9170-z. Epub 2009 Apr 9.

PMID:
19357993
16.

Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption.

Abdullah N, Chase HA.

Biotechnol Bioeng. 2005 Nov 20;92(4):501-13.

PMID:
16080185
17.

Expression, refolding, and characterization of recombinant thrombopoietin/stem cell factor fusion protein in Escherichia coli.

Zang Y, Zhang X, Jiang X, Li H, Zhu J, Zhang C, Peng W, Qin J.

Appl Microbiol Biotechnol. 2007 Mar;74(4):836-42. Epub 2006 Nov 23.

PMID:
17123074
18.

Expression of bioactive recombinant GSLL-39, a variant of human antimicrobial peptide LL-37, in Escherichia coli.

Yang YH, Zheng GG, Li G, Zhang XJ, Cao ZY, Rao Q, Wu KF.

Protein Expr Purif. 2004 Sep;37(1):229-35.

PMID:
15294303
19.

One-step on-column purification and refolding of a single-chain variable fragment (scFv) antibody against tumour necrosis factor alpha.

Liu M, Wang X, Yin C, Zhang Z, Lin Q, Zhen Y, Huang H.

Biotechnol Appl Biochem. 2006 Mar;43(Pt 3):137-45.

PMID:
16293104
20.

Improved expression of a soluble single chain antibody fusion protein containing tumor necrosis factor in Escherichia coli.

Kim S, Cheung LH, Zhang W, Rosenblum MG.

Appl Microbiol Biotechnol. 2007 Nov;77(1):99-106. Epub 2007 Aug 17.

PMID:
17703297
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