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Items: 1 to 20 of 106

1.

A rapid and direct method for the determination of active site accessibility in proteins based on ESI-MS and active site titrations.

O'Farrell N, Kreiner M, Moore BD, Parker MC.

Biotechnol Bioeng. 2006 Nov 5;95(4):767-71.

PMID:
16917861
2.

Temperature-gradient gel electrophoresis for analysis and screening of thermostable proteases.

Sättler A, Riesner D.

Electrophoresis. 1993 Aug;14(8):782-8.

PMID:
8404822
3.

Active-site titration of serine proteases using a fluoride ion selective electrode and sulfonyl fluoride inhibitors.

Hsia CY, Ganshaw G, Paech C, Murray CJ.

Anal Biochem. 1996 Nov 15;242(2):221-7.

PMID:
8937565
4.

Reactive-electrospray-assisted laser desorption/ionization for characterization of peptides and proteins.

Peng IX, Ogorzalek Loo RR, Shiea J, Loo JA.

Anal Chem. 2008 Sep 15;80(18):6995-7003. doi: 10.1021/ac800870c. Epub 2008 Aug 7.

PMID:
18683952
5.

Determination of active enzyme concentration using activity-based probes and direct mass spectrometric readout.

Bovet C, Zenobi R.

Anal Biochem. 2008 Feb 15;373(2):380-2. Epub 2007 Nov 6.

PMID:
18068109
6.

Online microwave D-cleavage LC-ESI-MS/MS of intact proteins: site-specific cleavages at aspartic acid residues and disulfide bonds.

Hauser NJ, Basile F.

J Proteome Res. 2008 Mar;7(3):1012-26. doi: 10.1021/pr700596e. Epub 2008 Jan 17.

PMID:
18198820
7.

Which electrospray-based ionization method best reflects protein-ligand interactions found in solution? a comparison of ESI, nanoESI, and ESSI for the determination of dissociation constants with mass spectrometry.

Jecklin MC, Touboul D, Bovet C, Wortmann A, Zenobi R.

J Am Soc Mass Spectrom. 2008 Mar;19(3):332-43. Epub 2007 Nov 19. Erratum in: J Am Soc Mass Spectrom. 2008 Aug;19(8):1237.

8.

Top-down ESI-ECD-FT-ICR mass spectrometry localizes noncovalent protein-ligand binding sites.

Xie Y, Zhang J, Yin S, Loo JA.

J Am Chem Soc. 2006 Nov 15;128(45):14432-3.

PMID:
17090006
9.

Analysis of proteins in the spent culture medium of Lupinus albus by electrospray ionisation tandem mass spectrometry.

Schwend T, Redwanz I, Ruppert T, Szenthe A, Wink M.

J Chromatogr A. 2003 Aug 15;1009(1-2):105-10.

PMID:
13677650
10.

Active-site titration of serine proteases in organic solvents.

Wangikar PP, Carmichael D, Clark DS, Dordick JS.

Biotechnol Bioeng. 1996 May 5;50(3):329-35.

PMID:
18626960
11.

Binding constant determination of high-affinity protein-ligand complexes by electrospray ionization mass spectrometry and ligand competition.

Wortmann A, Jecklin MC, Touboul D, Badertscher M, Zenobi R.

J Mass Spectrom. 2008 May;43(5):600-8.

PMID:
18074334
13.

Quantifying carbohydrate-protein interactions by electrospray ionization mass spectrometry analysis.

El-Hawiet A, Kitova EN, Klassen JS.

Biochemistry. 2012 May 29;51(21):4244-53. doi: 10.1021/bi300436x. Epub 2012 May 17.

PMID:
22564104
14.

Non-denaturing electrospray ionisation-mass spectrometry reveals ligand selectivity in histamine-binding protein RaHBP2.

Oldham NJ, Lissina O, Nunn MA, Paesen GC.

Org Biomol Chem. 2003 Nov 7;1(21):3645-6.

PMID:
14649894
15.

Time of flight versus ion trap MS coupled to CE to analyse intact proteins.

Erny GL, León C, Marina ML, Cifuentes A.

J Sep Sci. 2008 Jun;31(10):1810-8. doi: 10.1002/jssc.200700651.

PMID:
18481325
16.
17.

Capillary electrophoresis-mass spectrometry for the analysis of intact proteins.

Haselberg R, de Jong GJ, Somsen GW.

J Chromatogr A. 2007 Aug 3;1159(1-2):81-109. Epub 2007 May 18. Review.

PMID:
17560583
18.

Electrospray ionization mass spectrometry in enzymology: uncovering the mechanisms of two-substrate reactions.

Shipovskov S, Reimann CT.

Analyst. 2007 May;132(5):397-402. Epub 2007 Mar 22. Review.

PMID:
17471382
19.

In-gel digestion of proteins for MALDI-MS fingerprint mapping.

Jiménez CR, Huang L, Qiu Y, Burlingame AL.

Curr Protoc Protein Sci. 2001 May;Chapter 16:Unit 16.4. doi: 10.1002/0471140864.ps1604s14.

PMID:
18429131
20.

Electrospray-ionization mass spectrometry as a tool for fast screening of protein structural properties.

Grandori R, Santambrogio C, Brocca S, Invernizzi G, Lotti M.

Biotechnol J. 2009 Jan;4(1):73-87. doi: 10.1002/biot.200800250. Review.

PMID:
19156745
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