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Items: 1 to 20 of 172

1.

Directed evolution of DNA polymerase, RNA polymerase and reverse transcriptase activity in a single polypeptide.

Ong JL, Loakes D, Jaroslawski S, Too K, Holliger P.

J Mol Biol. 2006 Aug 18;361(3):537-50. Epub 2006 Jul 5.

PMID:
16859707
2.

Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase.

Xia G, Chen L, Sera T, Fa M, Schultz PG, Romesberg FE.

Proc Natl Acad Sci U S A. 2002 May 14;99(10):6597-602.

3.

The steric gate of DNA polymerase ╬╣ regulates ribonucleotide incorporation and deoxyribonucleotide fidelity.

Donigan KA, McLenigan MP, Yang W, Goodman MF, Woodgate R.

J Biol Chem. 2014 Mar 28;289(13):9136-45. doi: 10.1074/jbc.M113.545442. Epub 2014 Feb 14.

4.

A mutant T7 RNA polymerase as a DNA polymerase.

Sousa R, Padilla R.

EMBO J. 1995 Sep 15;14(18):4609-21.

5.

Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution.

Ghadessy FJ, Ramsay N, Boudsocq F, Loakes D, Brown A, Iwai S, Vaisman A, Woodgate R, Holliger P.

Nat Biotechnol. 2004 Jun;22(6):755-9. Epub 2004 May 23.

PMID:
15156154
6.

Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA.

Kostyuk DA, Dragan SM, Lyakhov DL, Rechinsky VO, Tunitskaya VL, Chernov BK, Kochetkov SN.

FEBS Lett. 1995 Aug 7;369(2-3):165-8.

7.

Catalytic editing properties of DNA polymerases.

Canard B, Cardona B, Sarfati RS.

Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):10859-63.

8.

Choosing the right sugar: how polymerases select a nucleotide substrate.

Joyce CM.

Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1619-22. No abstract available.

9.

One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase.

Kranaster R, Drum M, Engel N, Weidmann M, Hufert FT, Marx A.

Biotechnol J. 2010 Feb;5(2):224-31. doi: 10.1002/biot.200900200.

PMID:
20108275
11.

Chimeric thermostable DNA polymerases with reverse transcriptase and attenuated 3'-5' exonuclease activity.

Sch├Ânbrunner NJ, Fiss EH, Budker O, Stoffel S, Sigua CL, Gelfand DH, Myers TW.

Biochemistry. 2006 Oct 24;45(42):12786-95.

PMID:
17042497
12.
13.

Conferring RNA polymerase activity to a DNA polymerase: a single residue in reverse transcriptase controls substrate selection.

Gao G, Orlova M, Georgiadis MM, Hendrickson WA, Goff SP.

Proc Natl Acad Sci U S A. 1997 Jan 21;94(2):407-11.

15.

Multiple amino acid substitutions allow DNA polymerases to synthesize RNA.

Patel PH, Loeb LA.

J Biol Chem. 2000 Dec 22;275(51):40266-72.

16.
17.

New substrates of DNA polymerases.

Victorova L, Sosunov V, Skoblov A, Shipytsin A, Krayevsky A.

FEBS Lett. 1999 Jun 18;453(1-2):6-10.

18.

Enhanced ribonucleotide incorporation by an O-helix mutant of Thermus aquaticus DNA polymerase I.

Ogawa M, Tosaka A, Ito Y, Yoshida S, Suzuki M.

Mutat Res. 2001 Apr 4;485(3):197-207.

PMID:
11267831
19.

Learning from directed evolution: Thermus aquaticus DNA polymerase mutants with translesion synthesis activity.

Obeid S, Schnur A, Gloeckner C, Blatter N, Welte W, Diederichs K, Marx A.

Chembiochem. 2011 Jul 4;12(10):1574-80. doi: 10.1002/cbic.201000783. Epub 2011 Apr 8.

PMID:
21480455
20.

Polymerase activities and RNA structures in the atomic force microscope.

Hansma HG, Golan R, Hsieh W, Daubendiek SL, Kool ET.

J Struct Biol. 1999 Oct;127(3):240-7.

PMID:
10544049
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