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2.

Budding yeast GCN1 binds the GI domain to activate the eIF2alpha kinase GCN2.

Kubota H, Ota K, Sakaki Y, Ito T.

J Biol Chem. 2001 May 18;276(20):17591-6. Epub 2001 Feb 28.

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Evidence that GCN1 and GCN20, translational regulators of GCN4, function on elongating ribosomes in activation of eIF2alpha kinase GCN2.

Marton MJ, Vazquez de Aldana CR, Qiu H, Chakraburtty K, Hinnebusch AG.

Mol Cell Biol. 1997 Aug;17(8):4474-89.

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Mutations activating the yeast eIF-2 alpha kinase GCN2: isolation of alleles altering the domain related to histidyl-tRNA synthetases.

Ramirez M, Wek RC, Vazquez de Aldana CR, Jackson BM, Freeman B, Hinnebusch AG.

Mol Cell Biol. 1992 Dec;12(12):5801-15.

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YIH1 is an actin-binding protein that inhibits protein kinase GCN2 and impairs general amino acid control when overexpressed.

Sattlegger E, Swanson MJ, Ashcraft EA, Jennings JL, Fekete RA, Link AJ, Hinnebusch AG.

J Biol Chem. 2004 Jul 16;279(29):29952-62. Epub 2004 May 4.

13.

IMPACT, a protein preferentially expressed in the mouse brain, binds GCN1 and inhibits GCN2 activation.

Pereira CM, Sattlegger E, Jiang HY, Longo BM, Jaqueta CB, Hinnebusch AG, Wek RC, Mello LE, Castilho BA.

J Biol Chem. 2005 Aug 5;280(31):28316-23. Epub 2005 Jun 2.

14.

Gcn2 mediates Gcn4 activation in response to glucose stimulation or UV radiation not via GCN4 translation.

Marbach I, Licht R, Frohnmeyer H, Engelberg D.

J Biol Chem. 2001 May 18;276(20):16944-51. Epub 2001 Feb 28.

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Rapamycin-induced translational derepression of GCN4 mRNA involves a novel mechanism for activation of the eIF2 alpha kinase GCN2.

Kubota H, Obata T, Ota K, Sasaki T, Ito T.

J Biol Chem. 2003 Jun 6;278(23):20457-60. Epub 2003 Apr 2.

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Serine 577 is phosphorylated and negatively affects the tRNA binding and eIF2alpha kinase activities of GCN2.

Garcia-Barrio M, Dong J, Cherkasova VA, Zhang X, Zhang F, Ufano S, Lai R, Qin J, Hinnebusch AG.

J Biol Chem. 2002 Aug 23;277(34):30675-83. Epub 2002 Jun 17.

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