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Results: 1 to 20 of 123

1.

Fast and accurate method for quantitating E. coli host-cell DNA contamination in plasmid DNA preparations.

Smith GJ 3rd, Helf M, Nesbet C, Betita HA, Meek J, Ferre F.

Biotechniques. 1999 Mar;26(3):518-22, 524, 526.

PMID:
10090994
[PubMed - indexed for MEDLINE]
2.

Quantitation of host cell DNA contaminate in pharmaceutical-grade plasmid DNA using competitive polymerase chain reaction and enzyme-linked immunosorbent assay.

Lahijani R, Duhon M, Lusby E, Betita H, Marquet M.

Hum Gene Ther. 1998 May 20;9(8):1173-80.

PMID:
9625256
[PubMed - indexed for MEDLINE]
3.

Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products.

Martins SA, Prazeres DM, Cabral JM, Monteiro GA.

Anal Biochem. 2003 Nov 1;322(1):127-9. No abstract available.

PMID:
14705789
[PubMed - indexed for MEDLINE]
4.

16S rRNA gene probe quantitates residual host cell DNA in pharmaceutical-grade plasmid DNA.

Wang KY, Guo YJ, Sun SH, Shi K, Zhang S, Wang KH, Yi-Zhang, Chen ZH.

Vaccine. 2006 Mar 24;24(14):2656-61. Epub 2005 Dec 27.

PMID:
16472542
[PubMed - indexed for MEDLINE]
5.

Quantification of Escherichia coli genomic DNA contamination in recombinant protein preparations by polymerase chain reaction and affinity-based collection.

Gregory CA, Rigg GP, Illidge CM, Matthews RC.

Anal Biochem. 2001 Sep 1;296(1):114-21.

PMID:
11520039
[PubMed - indexed for MEDLINE]
6.

Development of specific and rapid detection of bacterial pathogens in dairy products by PCR.

Chotár M, Vidová B, Godány A.

Folia Microbiol (Praha). 2006;51(6):639-46.

PMID:
17455804
[PubMed - indexed for MEDLINE]
7.

Real-time PCR determination of Escherichia coli genomic DNA contamination in plasmid preparations.

Vilalta A, Whitlow V, Martin T.

Anal Biochem. 2002 Feb 1;301(1):151-3. No abstract available.

PMID:
11811979
[PubMed - indexed for MEDLINE]
8.

Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli.

Lee C, Kim J, Shin SG, Hwang S.

J Biotechnol. 2006 May 29;123(3):273-80. Epub 2006 Jan 18.

PMID:
16388869
[PubMed - indexed for MEDLINE]
9.

Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria.

Lee CL, Ow DS, Oh SK.

J Microbiol Methods. 2006 May;65(2):258-67. Epub 2005 Sep 21.

PMID:
16181694
[PubMed - indexed for MEDLINE]
10.

Study of the influence of plasmids on the arbitrary primer polymerase chain reaction fingerprint of Escherichia coli strains.

Elaichouni A, van Emmelo J, Claeys G, Verschraegen G, Verhelst R, Vaneechoutte M.

FEMS Microbiol Lett. 1994 Jan 15;115(2-3):335-9.

PMID:
8138145
[PubMed - indexed for MEDLINE]
11.

Reagent decontamination to eliminate false-positives in Escherichia coli qPCR.

Silkie SS, Tolcher MP, Nelson KL.

J Microbiol Methods. 2008 Mar;72(3):275-82. doi: 10.1016/j.mimet.2007.12.011. Epub 2007 Dec 31.

PMID:
18280599
[PubMed - indexed for MEDLINE]
12.

Inter- and intra-genomic heterogeneity of the intervening sequence in the 23S ribosomal RNA gene of Campylobacter jejuni and Campylobacter coli.

Meinersmann RJ, Phillips RW, Ladely SR.

Syst Appl Microbiol. 2009 Apr;32(2):91-100. doi: 10.1016/j.syapm.2008.12.004. Epub 2009 Feb 6.

PMID:
19201124
[PubMed - indexed for MEDLINE]
13.
14.

Identification and characterization of IS1 transposition in plasmid amplification mutants of E. coli clones producing DNA vaccines.

Prather KL, Edmonds MC, Herod JW.

Appl Microbiol Biotechnol. 2006 Dec;73(4):815-26. Epub 2006 Aug 29.

PMID:
16941177
[PubMed - indexed for MEDLINE]
15.

Establishment and analysis of specific DNA patterns in 16S-23S rRNA gene spacer regions for differentiating different bacteria.

Shang S, Fu J, Dong G, Hong W, Du L, Yu X.

Chin Med J (Engl). 2003 Jan;116(1):129-33.

PMID:
12667405
[PubMed - indexed for MEDLINE]
Free Article
16.

Simultaneous quantitative detection of Listeria spp. and Listeria monocytogenes using a duplex real-time PCR-based assay.

Rodríguez-Lázaro D, Hernández M, Pla M.

FEMS Microbiol Lett. 2004 Apr 15;233(2):257-67.

PMID:
15063494
[PubMed - indexed for MEDLINE]
17.

Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates.

Johnson TJ, Wannemuehler YM, Johnson SJ, Logue CM, White DG, Doetkott C, Nolan LK.

Appl Environ Microbiol. 2007 Mar;73(6):1976-83. Epub 2007 Feb 2. Erratum in: Appl Environ Microbiol. 2007 Jun;73(11):3768.

PMID:
17277222
[PubMed - indexed for MEDLINE]
Free PMC Article
18.

Cancer gene therapy using plasmid DNA: purification of DNA for human clinical trials.

Horn NA, Meek JA, Budahazi G, Marquet M.

Hum Gene Ther. 1995 May;6(5):565-73.

PMID:
7578394
[PubMed - indexed for MEDLINE]
19.

Development of a rapid quantitative PCR assay for direct detection and quantification of culturable and non-culturable Escherichia coli from agriculture watersheds.

Khan IU, Gannon V, Kent R, Koning W, Lapen DR, Miller J, Neumann N, Phillips R, Robertson W, Topp E, van Bochove E, Edge TA.

J Microbiol Methods. 2007 Jun;69(3):480-8. Epub 2007 Mar 12.

PMID:
17433480
[PubMed - indexed for MEDLINE]
20.

Purification of plasmids by triplex affinity interaction.

Schluep T, Cooney CL.

Nucleic Acids Res. 1998 Oct 1;26(19):4524-8.

PMID:
9742258
[PubMed - indexed for MEDLINE]
Free PMC Article

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