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Substrate Locking Promotes Dimer-Dimer Docking of an Enzyme Antibiotic Target.
Atkinson SC, Dogovski C, Wood K, Griffin MDW, Gorman MA, Hor L, Reboul CF, Buckle AM, Wuttke J, Parker MW, Dobson RCJ, Perugini MA. Atkinson SC, et al. Structure. 2018 Jul 3;26(7):948-959.e5. doi: 10.1016/j.str.2018.04.014. Epub 2018 May 24. Structure. 2018. PMID: 29804823 Free article.
We demonstrate that the substrate, pyruvate, stabilizes the more active dimer-of-dimers or tetrameric form. Surprisingly, there is little difference between the crystal structures of apo and substrate-bound enzyme, suggesting protein dynamics may be important. ...We theref …
We demonstrate that the substrate, pyruvate, stabilizes the more active dimer-of-dimers or tetrameric form. Surprisingly, there is little di …
Structure and function of the two-component flavin-dependent methanesulfinate monooxygenase within bacterial sulfur assimilation.
Soule J, Gnann AD, Gonzalez R, Parker MJ, McKenna KC, Nguyen SV, Phan NT, Wicht DK, Dowling DP. Soule J, et al. Biochem Biophys Res Commun. 2020 Jan 29;522(1):107-112. doi: 10.1016/j.bbrc.2019.11.008. Epub 2019 Nov 18. Biochem Biophys Res Commun. 2020. PMID: 31753487
We employed crystallographic and biochemical studies to identify the function of MsuC from Pseudomonas fluorescens. The crystal structure of MsuC adopts the acyl-CoA dehydrogenase fold with putative binding sites for flavin and MSI(-), and functional assays of MsuC in the …
We employed crystallographic and biochemical studies to identify the function of MsuC from Pseudomonas fluorescens. The crystal struc …
Copper(II) complexes of cyclams with N-(2,2,2-trifluoroethyl)-aminoalkyl pendant arms as potential probes for 19F magnetic resonance imaging.
Kotková Z, Koucký F, Kotek J, Císařová I, Parker D, Hermann P. Kotková Z, et al. Dalton Trans. 2023 Feb 14;52(7):1861-1875. doi: 10.1039/d2dt03360g. Dalton Trans. 2023. PMID: 36448539
A number of structures of the complexes as well as of several organic precursors were determined by single-crystal X-ray diffraction analysis. Geometric parameters (especially distances between fluorine atoms and the central metal ion) were determined for each complex and …
A number of structures of the complexes as well as of several organic precursors were determined by single-crystal X-ray diffraction …
Imaging MALDI mass spectrometry using an oscillating capillary nebulizer matrix coating system and its application to analysis of lipids in brain from a mouse model of Tay-Sachs/Sandhoff disease.
Chen Y, Allegood J, Liu Y, Wang E, Cachón-Gonzalez B, Cox TM, Merrill AH Jr, Sullards MC. Chen Y, et al. Anal Chem. 2008 Apr 15;80(8):2780-8. doi: 10.1021/ac702350g. Epub 2008 Mar 4. Anal Chem. 2008. PMID: 18314967
This article describes the use of an oscillating capillary nebulizer (OCN) to spray small droplets of matrix aerosol onto the sample surface for improved matrix homogeneity, reduced crystal size, and controlled solvent effects. This system was then applied to the analysis …
This article describes the use of an oscillating capillary nebulizer (OCN) to spray small droplets of matrix aerosol onto the sample surface …
The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
Smith IW, d'Aquino AE, Coyle CW, Fedanov A, Parker ET, Denning G, Spencer HT, Lollar P, Doering CB, Spiegel PC Jr. Smith IW, et al. J Thromb Haemost. 2020 Jan;18(1):57-69. doi: 10.1111/jth.14621. Epub 2019 Sep 8. J Thromb Haemost. 2020. PMID: 31454152 Free PMC article.
METHODS: The structure of ET3i was characterized with X-ray crystallography and tandem mass spectrometry-based glycoproteomics. RESULTS: Here, we report the 3.2 A crystal structure of ET3i and characterize the distribution of N-linked glycans with LC-MS/MS gl …
METHODS: The structure of ET3i was characterized with X-ray crystallography and tandem mass spectrometry-based glycoproteomics. RESULTS: Her …
Severe Acute Respiratory Syndrome Coronavirus 2 Infection and Pregnancy in Sub-Saharan Africa: A 6-Country Retrospective Cohort Analysis.
Nachega JB, Sam-Agudu NA, Machekano RN, Rosenthal PJ, Schell S, de Waard L, Bekker A, Gachuno OW, Kinuthia J, Mwongeli N, Budhram S, Vannevel V, Somapillay P, Prozesky HW, Taljaard J, Parker A, Agyare E, Opoku AB, Makarfi AU, Abdullahi AM, Adirieje C, Ishoso DK, Pipo MT, Tshilanda MB, Bongo-Pasi Nswe C, Ditekemena J, Sigwadhi LN, Nyasulu PS, Hermans MP, Sekikubo M, Musoke P, Nsereko C, Agbeno EK, Yeboah MY, Umar LW, Ntakwinja M, Mukwege DM, Birindwa EK, Mushamuka SZ, Smith ER, Mills EJ, Otshudiema JO, Mbala-Kingebeni P, Tamfum JM, Zumla A, Tsegaye A, Mteta A, Sewankambo NK, Suleman F, Adejumo P, Anderson JR, Noormahomed EV, Deckelbaum RJ, Stringer JSA, Mukalay A, Taha TE, Fowler MG, Wasserheit JN, Masekela R, Mellors JW, Siedner MJ, Myer L, Kengne AP, Yotebieng M, Mofenson LM, Langenegger E; AFREhealth Research Collaboration on COVID-19 and Pregnancy. Nachega JB, et al. Clin Infect Dis. 2022 Nov 30;75(11):1950-1961. doi: 10.1093/cid/ciac294. Clin Infect Dis. 2022. PMID: 36130257 Free PMC article.
Structural and spectroscopic studies of a rare non-oxido V(v) complex crystallized from aqueous solution.
Leggett CJ, Parker BF, Teat SJ, Zhang Z, Dau PD, Lukens WW, Peterson SM, Cardenas AJP, Warner MG, Gibson JK, Arnold J, Rao L. Leggett CJ, et al. Chem Sci. 2016 Apr 1;7(4):2775-2786. doi: 10.1039/c5sc03958d. Epub 2016 Jan 14. Chem Sci. 2016. PMID: 28660055 Free PMC article.
Using (17)O-labelled vanadate as the starting material, concurrent (17)O/(51)V/(1)H/(13)C NMR, in conjunction with ESI-MS, unprecedentedly demonstrated the stepwise displacement of the oxido V[double bond, length as m-dash]O bonds by glutaroimide-dioxime and verified the e …
Using (17)O-labelled vanadate as the starting material, concurrent (17)O/(51)V/(1)H/(13)C NMR, in conjunction with ESI-MS, unpreceden …
Synthesis and characterisation of dimeric eight-coordinate lanthanide(III) complexes of a macrocyclic tribenzylphosphinate ligand.
Senanayake K, Thompson AL, Howard JA, Botta M, Parker D. Senanayake K, et al. Dalton Trans. 2006 Dec 7;(45):5423-8. doi: 10.1039/b612189f. Epub 2006 Sep 29. Dalton Trans. 2006. PMID: 17102868
The macrocyclic ligand 1,4,7,10-tetraazacyclododecane-1,4,7-triyl(methylenebenzyl-phosphinic acid) H3L3, has been prepared and its complexes with Eu, Gd and Tb(III) studied by NMR, relaxometry, luminescence and single crystal X-ray crystallography. In solution and in the …
The macrocyclic ligand 1,4,7,10-tetraazacyclododecane-1,4,7-triyl(methylenebenzyl-phosphinic acid) H3L3, has been prepared and its complexes …