[Molecular study of the mechanism of chromosomal translocation (15;17) in acute promyelocytic leukemia (APL)]

Yi Chuan Xue Bao. 1993;20(5):381-8.
[Article in Chinese]

Abstract

Molecular studies of chromosomal translocation (15;17) in acute promyelocytic leukemia (APL) have shown that retinoic acid receptor A (RARA) gene on chromosome 17 is juxtaposed to the PML gene on chromosome 15. This results in a PML-RARA chimeric gene. Our work has demonstrated that the PML breakpoints in APL patients are clustered in two limited regions, PML-bcr1 and PML-bcr2, separated from each other by about 10 kb. DNA sequence of PML-bcr1 and primary structure of the junctional region of reciprocal chromosomal translocation in a patient have been determined in this paper. Compared to those of two previously reported cases abroad, we found that the breakpoint may be situated in the topoisomerase II cleavage site. A working model has been proposed for the mechanism of DNA illegitimate recombination in t (15;17).

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosomes, Human, Pair 15*
  • Chromosomes, Human, Pair 17*
  • Humans
  • Leukemia, Promyelocytic, Acute / genetics*
  • Molecular Sequence Data
  • Translocation, Genetic*