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Items: 1 to 20 of 52

1.

Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.

Encell LP, Friedman Ohana R, Zimmerman K, Otto P, Vidugiris G, Wood MG, Los GV, McDougall MG, Zimprich C, Karassina N, Learish RD, Hurst R, Hartnett J, Wheeler S, Stecha P, English J, Zhao K, Mendez J, Benink HA, Murphy N, Daniels DL, Slater MR, Urh M, Darzins A, Klaubert DH, Bulleit RF, Wood KV.

Curr Chem Genomics. 2012;6:55-71. doi: 10.2174/1875397301206010055. Epub 2012 Oct 5.

2.

Triple-color super-resolution imaging of live cells: resolving submicroscopic receptor organization in the plasma membrane.

Wilmes S, Staufenbiel M, Lisse D, Richter CP, Beutel O, Busch KB, Hess ST, Piehler J.

Angew Chem Int Ed Engl. 2012 May 14;51(20):4868-71. doi: 10.1002/anie.201200853. Epub 2012 Apr 5.

PMID:
22488831
3.

Identification of hydrophobic tags for the degradation of stabilized proteins.

Tae HS, Sundberg TB, Neklesa TK, Noblin DJ, Gustafson JL, Roth AG, Raina K, Crews CM.

Chembiochem. 2012 Mar 5;13(4):538-41. doi: 10.1002/cbic.201100793. Epub 2012 Jan 23.

4.

Examining the complexity of human RNA polymerase complexes using HaloTag technology coupled to label free quantitative proteomics.

Daniels DL, Méndez J, Mosley AL, Ramisetty SR, Murphy N, Benink H, Wood KV, Urh M, Washburn MP.

J Proteome Res. 2012 Feb 3;11(2):564-75. doi: 10.1021/pr200459c. Epub 2012 Jan 3.

PMID:
22149079
5.

Reporter protein-targeted probes for magnetic resonance imaging.

Strauch RC, Mastarone DJ, Sukerkar PA, Song Y, Ipsaro JJ, Meade TJ.

J Am Chem Soc. 2011 Oct 19;133(41):16346-9. doi: 10.1021/ja206134b. Epub 2011 Sep 26.

6.

HaloTag: a novel reporter gene for positron emission tomography.

Hong H, Benink HA, Zhang Y, Yang Y, Uyeda HT, Engle JW, Severin GW, McDougall MG, Barnhart TE, Klaubert DH, Nickles RJ, Fan F, Cai W.

Am J Transl Res. 2011 Aug 15;3(4):392-403. Epub 2011 Jul 28.

7.

Identification of a lysosomal pathway that modulates glucocorticoid signaling and the inflammatory response.

He Y, Xu Y, Zhang C, Gao X, Dykema KJ, Martin KR, Ke J, Hudson EA, Khoo SK, Resau JH, Alberts AS, MacKeigan JP, Furge KA, Xu HE.

Sci Signal. 2011 Jul 5;4(180):ra44. doi: 10.1126/scisignal.2001450.

8.

Small-molecule hydrophobic tagging-induced degradation of HaloTag fusion proteins.

Neklesa TK, Tae HS, Schneekloth AR, Stulberg MJ, Corson TW, Sundberg TB, Raina K, Holley SA, Crews CM.

Nat Chem Biol. 2011 Jul 3;7(8):538-43. doi: 10.1038/nchembio.597.

9.

Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome.

Zhou ZP, Shimizu Y, Tadakuma H, Taguchi H, Ito K, Ueda T.

J Biochem. 2011 May;149(5):609-18. doi: 10.1093/jb/mvr010. Epub 2011 Jan 28.

PMID:
21278155
10.

Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells.

Takemoto K, Matsuda T, McDougall M, Klaubert DH, Hasegawa A, Los GV, Wood KV, Miyawaki A, Nagai T.

ACS Chem Biol. 2011 May 20;6(5):401-6. doi: 10.1021/cb100431e. Epub 2011 Jan 20.

PMID:
21226520
11.

HaloTag-based purification of functional human kinases from mammalian cells.

Ohana RF, Hurst R, Vidugiriene J, Slater MR, Wood KV, Urh M.

Protein Expr Purif. 2011 Apr;76(2):154-64. doi: 10.1016/j.pep.2010.11.014. Epub 2010 Dec 1.

PMID:
21129486
12.

Expression and purification of full-length mouse CARM1 from transiently transfected HEK293T cells using HaloTag technology.

Chumanov RS, Kuhn PA, Xu W, Burgess RR.

Protein Expr Purif. 2011 Apr;76(2):145-53. doi: 10.1016/j.pep.2010.11.010. Epub 2010 Nov 30.

13.

Superresolution imaging of targeted proteins in fixed and living cells using photoactivatable organic fluorophores.

Lee HL, Lord SJ, Iwanaga S, Zhan K, Xie H, Williams JC, Wang H, Bowman GR, Goley ED, Shapiro L, Twieg RJ, Rao J, Moerner WE.

J Am Chem Soc. 2010 Nov 3;132(43):15099-101. doi: 10.1021/ja1044192.

14.

A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays.

Hartzell DD, Trinklein ND, Mendez J, Murphy N, Aldred SF, Wood K, Urh M.

BMC Genomics. 2009 Oct 27;10:497. doi: 10.1186/1471-2164-10-497.

15.

Direct pH measurements by using subcellular targeting of 5(and 6-) carboxyseminaphthorhodafluor in mammalian cells.

Benink H, McDougall M, Klaubert D, Los G.

Biotechniques. 2009 Sep;47(3):769-74. doi: 10.2144/000113220.

16.

In vivo stable tumor-specific painting in various colors using dehalogenase-based protein-tag fluorescent ligands.

Kosaka N, Ogawa M, Choyke PL, Karassina N, Corona C, McDougall M, Lynch DT, Hoyt CC, Levenson RM, Los GV, Kobayashi H.

Bioconjug Chem. 2009 Jul;20(7):1367-74. doi: 10.1021/bc9001344.

17.

Protein-protein interaction studies on protein arrays: effect of detection strategies on signal-to-background ratios.

Hurst R, Hook B, Slater MR, Hartnett J, Storts DR, Nath N.

Anal Biochem. 2009 Sep 1;392(1):45-53. doi: 10.1016/j.ab.2009.05.028. Epub 2009 May 22.

PMID:
19464993
18.

HaloTag7: a genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification.

Ohana RF, Encell LP, Zhao K, Simpson D, Slater MR, Urh M, Wood KV.

Protein Expr Purif. 2009 Nov;68(1):110-20. doi: 10.1016/j.pep.2009.05.010. Epub 2009 May 21.

PMID:
19464373
19.

Regulation of the processivity and intracellular localization of Saccharomyces cerevisiae dynein by dynactin.

Kardon JR, Reck-Peterson SL, Vale RD.

Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5669-74. doi: 10.1073/pnas.0900976106. Epub 2009 Mar 17.

20.

In vivo labeling method using a genetic construct for nanoscale resolution microscopy.

Schröder J, Benink H, Dyba M, Los GV.

Biophys J. 2009 Jan;96(1):L01-3. doi: 10.1016/j.bpj.2008.09.032.

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