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Items: 1 to 20 of 24


Superfolder GFP is fluorescent in oxidizing environments when targeted via the Sec translocon.

Aronson DE, Costantini LM, Snapp EL.

Traffic. 2011 May;12(5):543-8. doi: 10.1111/j.1600-0854.2011.01168.x. Epub 2011 Feb 25.


Benchmarking membrane protein detergent stability for improving throughput of high-resolution X-ray structures.

Sonoda Y, Newstead S, Hu NJ, Alguel Y, Nji E, Beis K, Yashiro S, Lee C, Leung J, Cameron AD, Byrne B, Iwata S, Drew D.

Structure. 2011 Jan 12;19(1):17-25. doi: 10.1016/j.str.2010.12.001.


Rescue of misfolded proteins and stabilization by small molecules.

Stevens RC, Sancho J, Martinez A.

Methods Mol Biol. 2010;648:313-24. doi: 10.1007/978-1-60761-756-3_22.


Function of human Rh based on structure of RhCG at 2.1 A.

Gruswitz F, Chaudhary S, Ho JD, Schlessinger A, Pezeshki B, Ho CM, Sali A, Westhoff CM, Stroud RM.

Proc Natl Acad Sci U S A. 2010 May 25;107(21):9638-43. doi: 10.1073/pnas.1003587107. Epub 2010 May 10.


Expression, purification and in vitro functional reconstitution of the chemokine receptor CCR1.

Allen SJ, Ribeiro S, Horuk R, Handel TM.

Protein Expr Purif. 2009 Jul;66(1):73-81. doi: 10.1016/j.pep.2009.03.001. Epub 2009 Mar 9.


High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81.

Takayama H, Chelikani P, Reeves PJ, Zhang S, Khorana HG.

PLoS One. 2008 Jun 4;3(6):e2314. doi: 10.1371/journal.pone.0002314.


GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae.

Drew D, Newstead S, Sonoda Y, Kim H, von Heijne G, Iwata S.

Nat Protoc. 2008;3(5):784-98. doi: 10.1038/nprot.2008.44.


The funnel approach to the precrystallization production of membrane proteins.

Lewinson O, Lee AT, Rees DC.

J Mol Biol. 2008 Mar 14;377(1):62-73. doi: 10.1016/j.jmb.2007.12.059. Epub 2008 Jan 4.


GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function.

Rosenbaum DM, Cherezov V, Hanson MA, Rasmussen SG, Thian FS, Kobilka TS, Choi HJ, Yao XJ, Weis WI, Stevens RC, Kobilka BK.

Science. 2007 Nov 23;318(5854):1266-73. Epub 2007 Oct 25.


Heterologous GPCR expression: a bottleneck to obtaining crystal structures.

McCusker EC, Bane SE, O'Malley MA, Robinson AS.

Biotechnol Prog. 2007 May-Jun;23(3):540-7. Epub 2007 Mar 31. Review.


Manipulating phospholipids for crystallization of a membrane transport protein.

Guan L, Smirnova IN, Verner G, Nagamori S, Kaback HR.

Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1723-6. Epub 2006 Jan 30. Erratum in: Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):6073. Nagamoni, Shushi [corrected to Nagamori, Shushi].


Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells.

Gan L, Alexander JM, Wittelsberger A, Thomas B, Rosenblatt M.

Protein Expr Purif. 2006 May;47(1):296-302. Epub 2005 Dec 5.


Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.

Long SB, Campbell EB, Mackinnon R.

Science. 2005 Aug 5;309(5736):897-903. Epub 2005 Jul 7.


Recognition of transmembrane helices by the endoplasmic reticulum translocon.

Hessa T, Kim H, Bihlmaier K, Lundin C, Boekel J, Andersson H, Nilsson I, White SH, von Heijne G.

Nature. 2005 Jan 27;433(7024):377-81.


Membrane protein expression and production: effects of polyhistidine tag length and position.

Mohanty AK, Wiener MC.

Protein Expr Purif. 2004 Feb;33(2):311-25.


Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells.

Jain RK, Joyce PB, Molinete M, Halban PA, Gorr SU.

Biochem J. 2001 Dec 15;360(Pt 3):645-9.

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