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Results: 18

1.

Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome.

Gibson DG, Benders GA, Andrews-Pfannkoch C, Denisova EA, Baden-Tillson H, Zaveri J, Stockwell TB, Brownley A, Thomas DW, Algire MA, Merryman C, Young L, Noskov VN, Glass JI, Venter JC, Hutchison CA 3rd, Smith HO.

Science. 2008 Feb 29;319(5867):1215-20. doi: 10.1126/science.1151721. Epub 2008 Jan 24.

PMID:
18218864
[PubMed - indexed for MEDLINE]
Free Article
2.

Bottom-up genome assembly using the Bacillus subtilis genome vector.

Itaya M, Fujita K, Kuroki A, Tsuge K.

Nat Methods. 2008 Jan;5(1):41-3. Epub 2007 Dec 9.

PMID:
18066072
[PubMed - indexed for MEDLINE]
3.

Gene synthesis by circular assembly amplification.

Bang D, Church GM.

Nat Methods. 2008 Jan;5(1):37-9. Epub 2007 Nov 25.

PMID:
18037891
[PubMed - indexed for MEDLINE]
4.

PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae.

Akada R, Kitagawa T, Kaneko S, Toyonaga D, Ito S, Kakihara Y, Hoshida H, Morimura S, Kondo A, Kida K.

Yeast. 2006 Apr 15;23(5):399-405.

PMID:
16598691
[PubMed - indexed for MEDLINE]
5.

A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast.

Hirashima K, Iwaki T, Takegawa K, Giga-Hama Y, Tohda H.

Nucleic Acids Res. 2006 Jan 24;34(2):e11.

PMID:
16434698
[PubMed - indexed for MEDLINE]
Free PMC Article
6.

Two-step method for constructing unmarked insertions, deletions and allele substitutions in the yeast genome.

Gray M, Piccirillo S, Honigberg SM.

FEMS Microbiol Lett. 2005 Jul 1;248(1):31-6.

PMID:
15953696
[PubMed - indexed for MEDLINE]
7.

Site-specific genomic (SSG) and random domain-localized (RDL) mutagenesis in yeast.

Gray M, Kupiec M, Honigberg SM.

BMC Biotechnol. 2004 Apr 16;4:7.

PMID:
15090068
[PubMed - indexed for MEDLINE]
Free PMC Article
8.

Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae.

Ito-Harashima S, McCusker JH.

Yeast. 2004 Jan 15;21(1):53-61.

PMID:
14745782
[PubMed - indexed for MEDLINE]
9.

Chromosomal site-specific double-strand breaks are efficiently targeted for repair by oligonucleotides in yeast.

Storici F, Durham CL, Gordenin DA, Resnick MA.

Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14994-9. Epub 2003 Nov 20.

PMID:
14630945
[PubMed - indexed for MEDLINE]
Free PMC Article
10.

In vivo site-directed mutagenesis using oligonucleotides.

Storici F, Lewis LK, Resnick MA.

Nat Biotechnol. 2001 Aug;19(8):773-6.

PMID:
11479573
[PubMed - indexed for MEDLINE]
11.

A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance.

Toyn JH, Gunyuzlu PL, White WH, Thompson LA, Hollis GF.

Yeast. 2000 Apr;16(6):553-60.

PMID:
10790693
[PubMed - indexed for MEDLINE]
12.

Factors affecting inverted repeat stimulation of recombination and deletion in Saccharomyces cerevisiae.

Lobachev KS, Shor BM, Tran HT, Taylor W, Keen JD, Resnick MA, Gordenin DA.

Genetics. 1998 Apr;148(4):1507-24.

PMID:
9560370
[PubMed - indexed for MEDLINE]
Free PMC Article
13.

Repair by recombination of DNA containing a palindromic sequence.

Leach DR, Okely EA, Pinder DJ.

Mol Microbiol. 1997 Nov;26(3):597-606.

PMID:
9402028
[PubMed - indexed for MEDLINE]
14.

Inverted DNA repeats: a source of eukaryotic genomic instability.

Gordenin DA, Lobachev KS, Degtyareva NP, Malkova AL, Perkins E, Resnick MA.

Mol Cell Biol. 1993 Sep;13(9):5315-22.

PMID:
8395002
[PubMed - indexed for MEDLINE]
Free PMC Article
15.

New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.

Wach A, Brachat A, Pöhlmann R, Philippsen P.

Yeast. 1994 Dec;10(13):1793-808.

PMID:
7747518
[PubMed - indexed for MEDLINE]
16.

A method for performing precise alterations in the yeast genome using a recycable selectable marker.

Längle-Rouault F, Jacobs E.

Nucleic Acids Res. 1995 Aug 11;23(15):3079-81. No abstract available.

PMID:
7659536
[PubMed - indexed for MEDLINE]
Free PMC Article
17.

Three Tn10-associated excision events: relationship to transposition and role of direct and inverted repeats.

Foster TJ, Lundblad V, Hanley-Way S, Halling SM, Kleckner N.

Cell. 1981 Jan;23(1):215-27.

PMID:
6260376
[PubMed - indexed for MEDLINE]
18.

Replacement of chromosome segments with altered DNA sequences constructed in vitro.

Scherer S, Davis RW.

Proc Natl Acad Sci U S A. 1979 Oct;76(10):4951-5.

PMID:
388424
[PubMed - indexed for MEDLINE]
Free PMC Article

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