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Results: 1 to 20 of 133

1.

Isotopic labeling of heterologous proteins in the yeast Pichia pastoris and Kluyveromyces lactis.

Sugiki T, Ichikawa O, Miyazawa-Onami M, Shimada I, Takahashi H.

Methods Mol Biol. 2012;831:19-36. doi: 10.1007/978-1-61779-480-3_2.

PMID:
22167666
[PubMed - indexed for MEDLINE]
2.

Stable isotope labeling of protein by Kluyveromyces lactis for NMR study.

Sugiki T, Shimada I, Takahashi H.

J Biomol NMR. 2008 Nov;42(3):159-62. doi: 10.1007/s10858-008-9276-9. Epub 2008 Oct 1.

PMID:
18827973
[PubMed - indexed for MEDLINE]
3.

Isotopic labeling of recombinant proteins from the methylotrophic yeast Pichia pastoris.

Pickford AR, O'Leary JM.

Methods Mol Biol. 2004;278:17-33.

PMID:
15317988
[PubMed - indexed for MEDLINE]
4.

Production of isotopically labeled heterologous proteins in non-E. coli prokaryotic and eukaryotic cells.

Takahashi H, Shimada I.

J Biomol NMR. 2010 Jan;46(1):3-10. doi: 10.1007/s10858-009-9377-0. Epub 2009 Sep 29.

PMID:
19787297
[PubMed - indexed for MEDLINE]
5.

Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation.

Wood MJ, Komives EA.

J Biomol NMR. 1999 Feb;13(2):149-59.

PMID:
10070756
[PubMed - indexed for MEDLINE]
6.

Perdeuteration and methyl-selective (1)H, (13)C-labeling by using a Kluyveromyces lactis expression system.

Miyazawa-Onami M, Takeuchi K, Takano T, Sugiki T, Shimada I, Takahashi H.

J Biomol NMR. 2013 Nov;57(3):297-304. doi: 10.1007/s10858-013-9789-8. Epub 2013 Oct 22.

PMID:
24146206
[PubMed - indexed for MEDLINE]
7.

Efficient 13C/15N double labeling of the avirulence protein AVR4 in a methanol-utilizing strain (Mut+) of Pichia pastoris.

van den Burg HA, de Wit PJ, Vervoort J.

J Biomol NMR. 2001 Jul;20(3):251-61.

PMID:
11519748
[PubMed - indexed for MEDLINE]
8.

Expression of deuterium-isotope-labelled protein in the yeast pichia pastoris for NMR studies.

Morgan WD, Kragt A, Feeney J.

J Biomol NMR. 2000 Aug;17(4):337-47.

PMID:
11014598
[PubMed - indexed for MEDLINE]
9.

Isotope labeling in mammalian cells.

Dutta A, Saxena K, Schwalbe H, Klein-Seetharaman J.

Methods Mol Biol. 2012;831:55-69. doi: 10.1007/978-1-61779-480-3_4.

PMID:
22167668
[PubMed - indexed for MEDLINE]
Free PMC Article
10.

Expression of the GM2-activator protein in the methylotrophic yeast Pichia pastoris, purification, isotopic labeling, and biophysical characterization.

Wendeler M, Hoernschemeyer J, John M, Werth N, Schoeniger M, Lemm T, Hartmann R, Kessler H, Sandhoff K.

Protein Expr Purif. 2004 Mar;34(1):147-57.

PMID:
14766311
[PubMed - indexed for MEDLINE]
11.

A novel bacterial expression method with optimized parameters for very high yield production of triple-labeled proteins.

Murray V, Huang Y, Chen J, Wang J, Li Q.

Methods Mol Biol. 2012;831:1-18. doi: 10.1007/978-1-61779-480-3_1.

PMID:
22167665
[PubMed - indexed for MEDLINE]
12.

Isotopic double-labeling of two honeybee odorant-binding proteins secreted by the methylotrophic yeast Pichia pastoris.

Briand L, Lescop E, B├ęzirard V, Birlirakis N, Huet JC, Henry C, Guittet E, Pernollet JC.

Protein Expr Purif. 2001 Oct;23(1):167-74.

PMID:
11570859
[PubMed - indexed for MEDLINE]
13.

Optimized procedure to generate heavy isotope and selenomethionine-labeled proteins for structure determination using Escherichia coli-based expression systems.

Li Z, Nimtz M, Rinas U.

Appl Microbiol Biotechnol. 2011 Nov;92(4):823-33. doi: 10.1007/s00253-011-3603-x. Epub 2011 Oct 9.

PMID:
21983707
[PubMed - indexed for MEDLINE]
14.

Development of a high-level expression system for deuterium-labeled human serum albumin.

Tomida M, Kimura M, Kuwata K, Hayashi T, Okano Y, Era S.

Jpn J Physiol. 2003 Feb;53(1):65-9.

PMID:
12689360
[PubMed - indexed for MEDLINE]
15.

Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra.

Emami S, Fan Y, Munro R, Ladizhansky V, Brown LS.

J Biomol NMR. 2013 Feb;55(2):147-55. doi: 10.1007/s10858-013-9710-5. Epub 2013 Jan 24.

PMID:
23344971
[PubMed - indexed for MEDLINE]
16.

Isotope labeling strategies for the study of high-molecular-weight proteins by solution NMR spectroscopy.

Tugarinov V, Kanelis V, Kay LE.

Nat Protoc. 2006;1(2):749-54.

PMID:
17406304
[PubMed - indexed for MEDLINE]
17.

Improving cell-free protein synthesis for stable-isotope labeling.

Matsuda T, Koshiba S, Tochio N, Seki E, Iwasaki N, Yabuki T, Inoue M, Yokoyama S, Kigawa T.

J Biomol NMR. 2007 Mar;37(3):225-9. Epub 2007 Jan 20.

PMID:
17237976
[PubMed - indexed for MEDLINE]
18.

Expression, purification, and mass spectrometric analysis of 15N, 13C-labeled RGD-hirudin, expressed in Pichia pastoris, for NMR studies.

Huang Y, Zhang Y, Wu Y, Wang J, Liu X, Dai L, Wang L, Yu M, Mo W.

PLoS One. 2012;7(8):e42207. doi: 10.1371/journal.pone.0042207. Epub 2012 Aug 7.

PMID:
22879918
[PubMed - indexed for MEDLINE]
Free PMC Article
19.

Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production.

Daly R, Hearn MT.

J Mol Recognit. 2005 Mar-Apr;18(2):119-38. Review.

PMID:
15565717
[PubMed - indexed for MEDLINE]
20.

The yeast Kluyveromyces lactis as an efficient host for heterologous gene expression.

Swinkels BW, van Ooyen AJ, Bonekamp FJ.

Antonie Van Leeuwenhoek. 1993-1994;64(2):187-201. Review.

PMID:
8092859
[PubMed - indexed for MEDLINE]

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