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Results: 1 to 20 of 122

1.

Restriction enzyme-free construction of random gene mutagenesis libraries in Escherichia coli.

Pai JC, Entzminger KC, Maynard JA.

Anal Biochem. 2012 Feb 15;421(2):640-8. doi: 10.1016/j.ab.2011.11.009. Epub 2011 Nov 18.

PMID:
22155067
[PubMed - indexed for MEDLINE]
Free PMC Article
2.

Thermostable DNA ligase-mediated PCR production of circular plasmid (PPCP) and its application in directed evolution via in situ error-prone PCR.

Le Y, Chen H, Zagursky R, Wu JH, Shao W.

DNA Res. 2013 Aug;20(4):375-82. doi: 10.1093/dnares/dst016. Epub 2013 Apr 30.

PMID:
23633530
[PubMed - indexed for MEDLINE]
Free PMC Article
3.

Polishing the craft of genetic diversity creation in directed evolution.

Tee KL, Wong TS.

Biotechnol Adv. 2013 Dec;31(8):1707-21. doi: 10.1016/j.biotechadv.2013.08.021. Epub 2013 Sep 6. Review.

PMID:
24012599
[PubMed - indexed for MEDLINE]
Free Article
4.

Easy preparation of a large-size random gene mutagenesis library in Escherichia coli.

You C, Percival Zhang YH.

Anal Biochem. 2012 Sep 1;428(1):7-12. doi: 10.1016/j.ab.2012.05.022. Epub 2012 May 30.

PMID:
22659340
[PubMed - indexed for MEDLINE]
5.

Improvements to the Kunkel mutagenesis protocol for constructing primary and secondary phage-display libraries.

Huang R, Fang P, Kay BK.

Methods. 2012 Sep;58(1):10-7. doi: 10.1016/j.ymeth.2012.08.008. Epub 2012 Aug 30.

PMID:
22959950
[PubMed - indexed for MEDLINE]
Free PMC Article
6.

AXM mutagenesis: an efficient means for the production of libraries for directed evolution of proteins.

Holland EG, Buhr DL, Acca FE, Alderman D, Bovat K, Busygina V, Kay BK, Weiner MP, Kiss MM.

J Immunol Methods. 2013 Aug 30;394(1-2):55-61. doi: 10.1016/j.jim.2013.05.003. Epub 2013 May 13.

PMID:
23680235
[PubMed - indexed for MEDLINE]
7.

Random mutagenesis strategies for construction of large and diverse clone libraries of mutated DNA fragments.

Chusacultanachai S, Yuthavong Y.

Methods Mol Biol. 2004;270:319-34.

PMID:
15153637
[PubMed - indexed for MEDLINE]
8.

Combinatorial engineering to enhance amylosucrase performance: construction, selection, and screening of variant libraries for increased activity.

van der Veen BA, Potocki-Véronèse G, Albenne C, Joucla G, Monsan P, Remaud-Simeon M.

FEBS Lett. 2004 Feb 27;560(1-3):91-7.

PMID:
14988004
[PubMed - indexed for MEDLINE]
9.

Construction and analysis of randomized protein-encoding libraries using error-prone PCR.

Hanson-Manful P, Patrick WM.

Methods Mol Biol. 2013;996:251-67. doi: 10.1007/978-1-62703-354-1_15.

PMID:
23504429
[PubMed - indexed for MEDLINE]
10.

Random mutagenesis by whole-plasmid PCR amplification.

Parikh A, Guengerich FP.

Biotechniques. 1998 Mar;24(3):428-31.

PMID:
9526653
[PubMed - indexed for MEDLINE]
11.

Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display.

Deng SJ, MacKenzie CR, Sadowska J, Michniewicz J, Young NM, Bundle DR, Narang SA.

J Biol Chem. 1994 Apr 1;269(13):9533-8.

PMID:
8144539
[PubMed - indexed for MEDLINE]
Free Article
12.

MEGAWHOP cloning: a method of creating random mutagenesis libraries via megaprimer PCR of whole plasmids.

Miyazaki K.

Methods Enzymol. 2011;498:399-406. doi: 10.1016/B978-0-12-385120-8.00017-6.

PMID:
21601687
[PubMed - indexed for MEDLINE]
13.

PCR-based strategy for construction of multi-site-saturation mutagenic expression library.

Wang J, Zhang S, Tan H, Zhao ZK.

J Microbiol Methods. 2007 Dec;71(3):225-30. Epub 2007 Sep 11.

PMID:
17936385
[PubMed - indexed for MEDLINE]
14.

Increased antibody expression from Escherichia coli through wobble-base library mutagenesis by enzymatic inverse PCR.

Stemmer WP, Morris SK, Kautzer CR, Wilson BS.

Gene. 1993 Jan 15;123(1):1-7.

PMID:
8422991
[PubMed - indexed for MEDLINE]
15.

Whole plasmid mutagenic PCR for directed protein evolution.

Matsumura I, Rowe LA.

Biomol Eng. 2005 Jun;22(1-3):73-9.

PMID:
15857786
[PubMed - indexed for MEDLINE]
16.
17.

Exploring the potential of megaprimer PCR in conjunction with orthogonal array design for mutagenesis library construction.

Tang L, Zheng K, Liu Y, Zheng H, Wang H, Song C, Zhou H.

Biotechnol Appl Biochem. 2013 Mar-Apr;60(2):190-5. doi: 10.1002/bab.1065. Epub 2013 Mar 15.

PMID:
23586485
[PubMed - indexed for MEDLINE]
18.

Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I.

Camps M, Naukkarinen J, Johnson BP, Loeb LA.

Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9727-32. Epub 2003 Aug 8.

PMID:
12909725
[PubMed - indexed for MEDLINE]
Free PMC Article
19.

The "Bringer" strategy: a very fast and highly efficient method for construction of mutant libraries by error-prone polymerase chain reaction of ring-closed plasmids.

Bichet A, Bureik M, Lenz N, Bernhardt R.

Appl Biochem Biotechnol. 2004 May;117(2):115-22.

PMID:
15159555
[PubMed - indexed for MEDLINE]
20.

Production of a vector to facilitate DNA mutagenesis and recombination.

Jones DH, Riley AN, Winistorfer SC.

Biotechniques. 1994 Apr;16(4):694-701.

PMID:
8024792
[PubMed - indexed for MEDLINE]

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