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Results: 1 to 20 of 110

Similar articles for PubMed (Select 21460824)

1.

A genome-scale shRNA resource for transgenic RNAi in Drosophila.

Ni JQ, Zhou R, Czech B, Liu LP, Holderbaum L, Yang-Zhou D, Shim HS, Tao R, Handler D, Karpowicz P, Binari R, Booker M, Brennecke J, Perkins LA, Hannon GJ, Perrimon N.

Nat Methods. 2011 May;8(5):405-7. doi: 10.1038/nmeth.1592. Epub 2011 Apr 3.

2.

Construction of simple and efficient DNA vector-based short hairpin RNA expression systems for specific gene silencing in mammalian cells.

Cheng TL, Chang WT.

Methods Mol Biol. 2007;408:223-41. doi: 10.1007/978-1-59745-547-3_13.

PMID:
18314586
3.

Generation of transgenic Drosophila expressing shRNAs in the miR-1 backbone.

Chang K, Marran K, Valentine A, Hannon GJ.

Cold Spring Harb Protoc. 2014 May 1;2014(5). pii: pdb.prot080762. doi: 10.1101/pdb.prot080762.

4.

Depleting gene activities in early Drosophila embryos with the "maternal-Gal4-shRNA" system.

Staller MV, Yan D, Randklev S, Bragdon MD, Wunderlich ZB, Tao R, Perkins LA, Depace AH, Perrimon N.

Genetics. 2013 Jan;193(1):51-61. doi: 10.1534/genetics.112.144915. Epub 2012 Oct 26.

5.

A direct comparison of strategies for combinatorial RNA interference.

Lambeth LS, Van Hateren NJ, Wilson SA, Nair V.

BMC Mol Biol. 2010 Oct 11;11:77. doi: 10.1186/1471-2199-11-77.

6.

DNA vector-based RNA interference to study gene function in cancer.

Stovall DB, Wan M, Zhang Q, Dubey P, Sui G.

J Vis Exp. 2012 Jun 4;(64):e4129. doi: 10.3791/4129.

7.

Characterisation and application of a bovine U6 promoter for expression of short hairpin RNAs.

Lambeth LS, Moore RJ, Muralitharan M, Dalrymple BP, McWilliam S, Doran TJ.

BMC Biotechnol. 2005 May 11;5:13.

8.

Organic small hairpin RNAs (OshR): a do-it-yourself platform for transgene-based gene silencing.

Zeng M, Kuzirian MS, Harper L, Paradis S, Nakayama T, Lau NC.

Methods. 2013 Sep 15;63(2):101-9. doi: 10.1016/j.ymeth.2013.05.007. Epub 2013 May 23.

9.

Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes.

Sarnova L, Malik R, Sedlacek R, Svoboda P.

J Negat Results Biomed. 2010 Oct 12;9:8. doi: 10.1186/1477-5751-9-8.

10.

Functional identification of optimized RNAi triggers using a massively parallel sensor assay.

Fellmann C, Zuber J, McJunkin K, Chang K, Malone CD, Dickins RA, Xu Q, Hengartner MO, Elledge SJ, Hannon GJ, Lowe SW.

Mol Cell. 2011 Mar 18;41(6):733-46. doi: 10.1016/j.molcel.2011.02.008. Epub 2011 Feb 25.

11.

A simplified miRNA-based gene silencing method for Drosophila melanogaster.

Haley B, Hendrix D, Trang V, Levine M.

Dev Biol. 2008 Sep 15;321(2):482-90. doi: 10.1016/j.ydbio.2008.06.015. Epub 2008 Jun 19.

12.

Online GESS: prediction of miRNA-like off-target effects in large-scale RNAi screen data by seed region analysis.

Yilmazel B, Hu Y, Sigoillot F, Smith JA, Shamu CE, Perrimon N, Mohr SE.

BMC Bioinformatics. 2014 Jun 17;15:192. doi: 10.1186/1471-2105-15-192.

13.

Vector-based RNAi approaches for stable, inducible and genome-wide screens.

Fewell GD, Schmitt K.

Drug Discov Today. 2006 Nov;11(21-22):975-82. Epub 2006 Sep 26. Review.

PMID:
17055406
14.

Generation of hairpin-based RNAi vectors for biological and therapeutic application.

Boudreau RL, Davidson BL.

Methods Enzymol. 2012;507:275-96. doi: 10.1016/B978-0-12-386509-0.00014-4. Review.

PMID:
22365779
15.

Effective gene knockdown in the Drosophila germline by artificial miRNA-mimicking siRNAs.

Wang H, Huang H, Chen D.

Methods Mol Biol. 2014;1093:225-34. doi: 10.1007/978-1-62703-694-8_18.

PMID:
24178569
16.
17.

Cross-species RNAi rescue platform in Drosophila melanogaster.

Kondo S, Booker M, Perrimon N.

Genetics. 2009 Nov;183(3):1165-73. doi: 10.1534/genetics.109.106567. Epub 2009 Aug 31.

18.

FlyPrimerBank: an online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents.

Hu Y, Sopko R, Foos M, Kelley C, Flockhart I, Ammeux N, Wang X, Perkins L, Perrimon N, Mohr SE.

G3 (Bethesda). 2013 Sep 4;3(9):1607-16. doi: 10.1534/g3.113.007021.

19.

A pipeline for the generation of shRNA transgenic mice.

Dow LE, Premsrirut PK, Zuber J, Fellmann C, McJunkin K, Miething C, Park Y, Dickins RA, Hannon GJ, Lowe SW.

Nat Protoc. 2012 Feb 2;7(2):374-93. doi: 10.1038/nprot.2011.446.

20.

Molecular mechanisms of RNA-triggered gene silencing machineries.

Li Z, Rana TM.

Acc Chem Res. 2012 Jul 17;45(7):1122-31. doi: 10.1021/ar200253u. Epub 2012 Feb 3.

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