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Items: 1 to 20 of 108

1.

CoESPRIT: a library-based construct screening method for identification and expression of soluble protein complexes.

An Y, Meresse P, Mas PJ, Hart DJ.

PLoS One. 2011 Feb 22;6(2):e16261. doi: 10.1371/journal.pone.0016261.

2.

ORF-selector ESPRIT: a second generation library screen for soluble protein expression employing precise open reading frame selection.

An Y, Yumerefendi H, Mas PJ, Chesneau A, Hart DJ.

J Struct Biol. 2011 Aug;175(2):189-97. doi: 10.1016/j.jsb.2011.04.004. Epub 2011 Apr 16.

PMID:
21515383
3.

A high-throughput immobilized bead screen for stable proteins and multi-protein complexes.

Lockard MA, Listwan P, Pedelacq JD, Cabantous S, Nguyen HB, Terwilliger TC, Waldo GS.

Protein Eng Des Sel. 2011 Jul;24(7):565-78. doi: 10.1093/protein/gzr021. Epub 2011 Jun 3.

4.

Strategies for the structural analysis of multi-protein complexes: lessons from the 3D-Repertoire project.

Collinet B, Friberg A, Brooks MA, van den Elzen T, Henriot V, Dziembowski A, Graille M, Durand D, Leulliot N, Saint André C, Lazar N, Sattler M, Séraphin B, van Tilbeurgh H.

J Struct Biol. 2011 Aug;175(2):147-58. doi: 10.1016/j.jsb.2011.03.018. Epub 2011 Apr 2.

PMID:
21463689
5.

Library-based methods for identification of soluble expression constructs.

Yumerefendi H, Desravines DC, Hart DJ.

Methods. 2011 Sep;55(1):38-43. doi: 10.1016/j.ymeth.2011.06.007. Epub 2011 Jun 23.

PMID:
21723393
6.

ESPRIT: an automated, library-based method for mapping and soluble expression of protein domains from challenging targets.

Yumerefendi H, Tarendeau F, Mas PJ, Hart DJ.

J Struct Biol. 2010 Oct;172(1):66-74. doi: 10.1016/j.jsb.2010.02.021. Epub 2010 Mar 4. Review.

PMID:
20206698
8.

A new bacterial co-expression system for over-expressing soluble protein and validating protein-protein interaction.

Zeng J, He ZG.

Methods Mol Biol. 2012;824:235-49. doi: 10.1007/978-1-61779-433-9_12.

PMID:
22160902
9.

Production of protein complexes via co-expression.

Kerrigan JJ, Xie Q, Ames RS, Lu Q.

Protein Expr Purif. 2011 Jan;75(1):1-14. doi: 10.1016/j.pep.2010.07.015. Epub 2010 Aug 6. Review.

PMID:
20692346
10.

Co-expression as a convenient method for the production and purification of core histones in bacteria.

Anderson M, Huh JH, Ngo T, Lee A, Hernandez G, Pang J, Perkins J, Dutnall RN.

Protein Expr Purif. 2010 Aug;72(2):194-204. doi: 10.1016/j.pep.2010.03.013. Epub 2010 Mar 27.

PMID:
20347990
11.

Expression of soluble, active fragments of the morphogenetic protein SpoIIE from Bacillus subtilis using a library-based construct screen.

Rawlings AE, Levdikov VM, Blagova E, Colledge VL, Mas PJ, Tunaley J, Vavrova L, Wilson KS, Barak I, Hart DJ, Wilkinson AJ.

Protein Eng Des Sel. 2010 Nov;23(11):817-25. doi: 10.1093/protein/gzq057. Epub 2010 Sep 3.

12.

Application of high-throughput methodologies to the expression of recombinant proteins in E. coli.

Peleg Y, Unger T.

Methods Mol Biol. 2008;426:197-208. doi: 10.1007/978-1-60327-058-8_12.

PMID:
18542865
13.

An efficient and generic strategy for producing soluble human proteins and domains in E. coli by screening construct libraries.

Cornvik T, Dahlroth SL, Magnusdottir A, Flodin S, Engvall B, Lieu V, Ekberg M, Nordlund P.

Proteins. 2006 Nov 1;65(2):266-73.

PMID:
16948159
14.

Expression of protein complexes using multiple Escherichia coli protein co-expression systems: a benchmarking study.

Busso D, Peleg Y, Heidebrecht T, Romier C, Jacobovitch Y, Dantes A, Salim L, Troesch E, Schuetz A, Heinemann U, Folkers GE, Geerlof A, Wilmanns M, Polewacz A, Quedenau C, Büssow K, Adamson R, Blagova E, Walton J, Cartwright JL, Bird LE, Owens RJ, Berrow NS, Wilson KS, Sussman JL, Perrakis A, Celie PH.

J Struct Biol. 2011 Aug;175(2):159-70. doi: 10.1016/j.jsb.2011.03.004. Epub 2011 Mar 5.

PMID:
21382497
15.

The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins.

Gräslund S, Sagemark J, Berglund H, Dahlgren LG, Flores A, Hammarström M, Johansson I, Kotenyova T, Nilsson M, Nordlund P, Weigelt J.

Protein Expr Purif. 2008 Apr;58(2):210-21. doi: 10.1016/j.pep.2007.11.008. Epub 2007 Nov 22.

PMID:
18171622
16.

Parallel methods for expression and purification.

Lesley SA.

Methods Enzymol. 2009;463:767-85. doi: 10.1016/S0076-6879(09)63041-X. Review.

PMID:
19892201
17.

Characterization of the interaction of full-length HIV-1 Vif protein with its key regulator CBFβ and CRL5 E3 ubiquitin ligase components.

Zhou X, Evans SL, Han X, Liu Y, Yu XF.

PLoS One. 2012;7(3):e33495. doi: 10.1371/journal.pone.0033495. Epub 2012 Mar 30.

18.

The pST44 polycistronic expression system for producing protein complexes in Escherichia coli.

Tan S, Kern RC, Selleck W.

Protein Expr Purif. 2005 Apr;40(2):385-95.

PMID:
15766881
19.

Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies.

Wang X, Campoli M, Ko E, Luo W, Ferrone S.

J Immunol Methods. 2004 Nov;294(1-2):23-35.

PMID:
15604013
20.
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