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Items: 1 to 20 of 158


Semi-automated library preparation for high-throughput DNA sequencing platforms.

Farias-Hesson E, Erikson J, Atkins A, Shen P, Davis RW, Scharfe C, Pourmand N.

J Biomed Biotechnol. 2010;2010:617469. doi: 10.1155/2010/617469. Epub 2010 Jun 8.


Increased throughput by parallelization of library preparation for massive sequencing.

Lundin S, Stranneheim H, Pettersson E, Klevebring D, Lundeberg J.

PLoS One. 2010 Apr 6;5(4):e10029. doi: 10.1371/journal.pone.0010029.


Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform.

Yegnasubramanian S.

Methods Enzymol. 2013;529:185-200. doi: 10.1016/B978-0-12-418687-3.00015-X.


Methods for generating shotgun and mixed shotgun/paired-end libraries for the 454 DNA sequencer.

Wiley G, Macmil S, Qu C, Wang P, Xing Y, White D, Li J, White JD, Domingo A, Roe BA.

Curr Protoc Hum Genet. 2009 Apr;Chapter 18:Unit18.1. doi: 10.1002/0471142905.hg1801s61.


Automated workflow for preparation of cDNA for cap analysis of gene expression on a single molecule sequencer.

Itoh M, Kojima M, Nagao-Sato S, Saijo E, Lassmann T, Kanamori-Katayama M, Kaiho A, Lizio M, Kawaji H, Carninci P, Forrest AR, Hayashizaki Y.

PLoS One. 2012;7(1):e30809. doi: 10.1371/journal.pone.0030809. Epub 2012 Jan 30.


"First generation" automated DNA sequencing technology.

Slatko BE, Kieleczawa J, Ju J, Gardner AF, Hendrickson CL, Ausubel FM.

Curr Protoc Mol Biol. 2011 Oct;Chapter 7:Unit7.2. doi: 10.1002/0471142727.mb0702s96.


Large scale library generation for high throughput sequencing.

Borgström E, Lundin S, Lundeberg J.

PLoS One. 2011 Apr 27;6(4):e19119. doi: 10.1371/journal.pone.0019119.


Preparing a re-sequencing DNA library of 2 cancer candidate genes using the ligation-by-amplification protocol by two PCR reactions.

Su Y, Lin L, Tian G, Chen C, Liu T, Xu X, Qi X, Zhang X, Yang H.

Sci China C Life Sci. 2009 May;52(5):483-91. doi: 10.1007/s11427-009-0066-8. Epub 2009 May 27.


Digital PCR provides sensitive and absolute calibration for high throughput sequencing.

White RA 3rd, Blainey PC, Fan HC, Quake SR.

BMC Genomics. 2009 Mar 19;10:116. doi: 10.1186/1471-2164-10-116. Erratum in: BMC Genomics. 2009;10:541.


Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation.

Quail MA, Gu Y, Swerdlow H, Mayho M.

Electrophoresis. 2012 Dec;33(23):3521-8. doi: 10.1002/elps.201200128. Epub 2012 Nov 12.


A microfluidic DNA library preparation platform for next-generation sequencing.

Kim H, Jebrail MJ, Sinha A, Bent ZW, Solberg OD, Williams KP, Langevin SA, Renzi RF, Van De Vreugde JL, Meagher RJ, Schoeniger JS, Lane TW, Branda SS, Bartsch MS, Patel KD.

PLoS One. 2013 Jul 22;8(7):e68988. doi: 10.1371/journal.pone.0068988. Print 2013.


An automated sample preparation system for large-scale DNA sequencing.

Marziali A, Willis TD, Federspiel NA, Davis RW.

Genome Res. 1999 May;9(5):457-62.


Multiplexed array-based and in-solution genomic enrichment for flexible and cost-effective targeted next-generation sequencing.

Harakalova M, Mokry M, Hrdlickova B, Renkens I, Duran K, van Roekel H, Lansu N, van Roosmalen M, de Bruijn E, Nijman IJ, Kloosterman WP, Cuppen E.

Nat Protoc. 2011 Nov 3;6(12):1870-86. doi: 10.1038/nprot.2011.396.


Illumina sequencing library preparation for highly multiplexed target capture and sequencing.

Meyer M, Kircher M.

Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.prot5448. doi: 10.1101/pdb.prot5448.


Targeted high throughput sequencing of a cancer-related exome subset by specific sequence capture with a fully automated microarray platform.

Summerer D, Schracke N, Wu H, Cheng Y, Bau S, Stähler CF, Stähler PF, Beier M.

Genomics. 2010 Apr;95(4):241-6. doi: 10.1016/j.ygeno.2010.01.006. Epub 2010 Feb 6.


Evaluation of a transposase protocol for rapid generation of shotgun high-throughput sequencing libraries from nanogram quantities of DNA.

Marine R, Polson SW, Ravel J, Hatfull G, Russell D, Sullivan M, Syed F, Dumas M, Wommack KE.

Appl Environ Microbiol. 2011 Nov;77(22):8071-9. doi: 10.1128/AEM.05610-11. Epub 2011 Sep 23.


Targeted multiplex next-generation sequencing: advances in techniques of mitochondrial and nuclear DNA sequencing for population genomics.

Hancock-Hanser BL, Frey A, Leslie MS, Dutton PH, Archer FI, Morin PA.

Mol Ecol Resour. 2013 Mar;13(2):254-68. doi: 10.1111/1755-0998.12059. Epub 2013 Jan 25.


A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454.

Lennon NJ, Lintner RE, Anderson S, Alvarez P, Barry A, Brockman W, Daza R, Erlich RL, Giannoukos G, Green L, Hollinger A, Hoover CA, Jaffe DB, Juhn F, McCarthy D, Perrin D, Ponchner K, Powers TL, Rizzolo K, Robbins D, Ryan E, Russ C, Sparrow T, Stalker J, Steelman S, Weiand M, Zimmer A, Henn MR, Nusbaum C, Nicol R.

Genome Biol. 2010;11(2):R15. doi: 10.1186/gb-2010-11-2-r15. Epub 2010 Feb 5.

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