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Systematic analysis of essential genes reveals important regulators of G protein signaling.

Cappell SD, Baker R, Skowyra D, Dohlman HG.

Mol Cell. 2010 Jun 11;38(5):746-57. doi: 10.1016/j.molcel.2010.05.026.


Pheromone-dependent destruction of the Tec1 transcription factor is required for MAP kinase signaling specificity in yeast.

Bao MZ, Schwartz MA, Cantin GT, Yates JR 3rd, Madhani HD.

Cell. 2004 Dec 29;119(7):991-1000.


A refined two-hybrid system reveals that SCF(Cdc4)-dependent degradation of Swi5 contributes to the regulatory mechanism of S-phase entry.

Kishi T, Ikeda A, Koyama N, Fukada J, Nagao R.

Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14497-502. doi: 10.1073/pnas.0806253105. Epub 2008 Sep 11.


The Cdc34/SCF ubiquitination complex mediates Saccharomyces cerevisiae cell wall integrity.

Varelas X, Stuart D, Ellison MJ, Ptak C.

Genetics. 2006 Dec;174(4):1825-39. Epub 2006 Oct 8.


F-box protein specificity for g1 cyclins is dictated by subcellular localization.

Landry BD, Doyle JP, Toczyski DP, Benanti JA.

PLoS Genet. 2012;8(7):e1002851. doi: 10.1371/journal.pgen.1002851. Epub 2012 Jul 26.


Nucleus-specific and cell cycle-regulated degradation of mitogen-activated protein kinase scaffold protein Ste5 contributes to the control of signaling competence.

Garrenton LS, Braunwarth A, Irniger S, Hurt E, Künzler M, Thorner J.

Mol Cell Biol. 2009 Jan;29(2):582-601. doi: 10.1128/MCB.01019-08. Epub 2008 Nov 10.


Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34.

Seol JH, Feldman RM, Zachariae W, Shevchenko A, Correll CC, Lyapina S, Chi Y, Galova M, Claypool J, Sandmeyer S, Nasmyth K, Deshaies RJ, Shevchenko A, Deshaies RJ.

Genes Dev. 1999 Jun 15;13(12):1614-26.


Dynamic ubiquitination of the mitogen-activated protein kinase kinase (MAPKK) Ste7 determines mitogen-activated protein kinase (MAPK) specificity.

Hurst JH, Dohlman HG.

J Biol Chem. 2013 Jun 28;288(26):18660-71. doi: 10.1074/jbc.M113.475707. Epub 2013 May 3.


The acidic tail of the Cdc34 ubiquitin-conjugating enzyme functions in both binding to and catalysis with ubiquitin ligase SCFCdc4.

Kleiger G, Hao B, Mohl DA, Deshaies RJ.

J Biol Chem. 2009 Dec 25;284(52):36012-23. doi: 10.1074/jbc.M109.058529. Epub 2009 Oct 29.


In vitro reconstitution of SCF substrate ubiquitination with purified proteins.

Petroski MD, Deshaies RJ.

Methods Enzymol. 2005;398:143-58.


Transferable domain in the G(1) cyclin Cln2 sufficient to switch degradation of Sic1 from the E3 ubiquitin ligase SCF(Cdc4) to SCF(Grr1).

Berset C, Griac P, Tempel R, La Rue J, Wittenberg C, Lanker S.

Mol Cell Biol. 2002 Jul;22(13):4463-76.


Guanine nucleotide-binding protein (Gα) endocytosis by a cascade of ubiquitin binding domain proteins is required for sustained morphogenesis and proper mating in yeast.

Dixit G, Baker R, Sacks CM, Torres MP, Dohlman HG.

J Biol Chem. 2014 May 23;289(21):15052-63. doi: 10.1074/jbc.M114.566117. Epub 2014 Apr 10.


The ubiquitin ligase SCFGrr1 is necessary for pheromone sensitivity in Saccharomyces cerevisiae.

Schweitzer K, Cocklin R, Garrett L, Desai F, Goebl M.

Yeast. 2005 May;22(7):553-64.


Regulation of neddylation and deneddylation of cullin1 in SCFSkp2 ubiquitin ligase by F-box protein and substrate.

Bornstein G, Ganoth D, Hershko A.

Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11515-20. Epub 2006 Jul 21.


Signal-induced disassembly of the SCF ubiquitin ligase complex by Cdc48/p97.

Yen JL, Flick K, Papagiannis CV, Mathur R, Tyrrell A, Ouni I, Kaake RM, Huang L, Kaiser P.

Mol Cell. 2012 Oct 26;48(2):288-97. doi: 10.1016/j.molcel.2012.08.015. Epub 2012 Sep 20.

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