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Items: 1 to 20 of 137

1.

Mutations in maltose-binding protein that alter affinity and solubility properties.

Walker IH, Hsieh PC, Riggs PD.

Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 10.1007/s00253-010-2696-y. Epub 2010 Jun 10.

3.

Positional effects of fusion partners on the yield and solubility of MBP fusion proteins.

Raran-Kurussi S, Keefe K, Waugh DS.

Protein Expr Purif. 2015 Jun;110:159-64. doi: 10.1016/j.pep.2015.03.004. Epub 2015 Mar 14.

PMID:
25782741
4.
5.

Cu/Zn incorporation during purification of soluble human EC-SOD from E. coli stabilizes proper disulfide bond formation.

Bae JY, Koo BK, Ryu HB, Song JA, Nguyen MT, Vu TT, Son YJ, Lee HK, Choe H.

Appl Biochem Biotechnol. 2013 Mar;169(5):1633-47. doi: 10.1007/s12010-012-0025-x. Epub 2013 Jan 19.

PMID:
23329142
6.
7.

Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner.

Sun P, Tropea JE, Waugh DS.

Methods Mol Biol. 2011;705:259-74. doi: 10.1007/978-1-61737-967-3_16. Review.

PMID:
21125392
9.

Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3).

Austin BP, Waugh DS.

Protein Expr Purif. 2012 Mar;82(1):116-24. doi: 10.1016/j.pep.2011.11.015. Epub 2011 Dec 14.

10.

Expression and purification of short hydrophobic elastin-like polypeptides with maltose-binding protein as a solubility tag.

Bataille L, Dieryck W, Hocquellet A, Cabanne C, Bathany K, Lecommandoux S, Garbay B, Garanger E.

Protein Expr Purif. 2015 Jun;110:165-71. doi: 10.1016/j.pep.2015.03.013. Epub 2015 Mar 27.

PMID:
25819942
11.

Genetic approach to the role of tryptophan residues in the activities and fluorescence of a bacterial periplasmic maltose-binding protein.

Martineau P, Szmelcman S, Spurlino JC, Quiocho FA, Hofnung M.

J Mol Biol. 1990 Jul 5;214(1):337-52.

PMID:
2196376
12.

Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag.

Duong-Ly KC, Gabelli SB.

Methods Enzymol. 2015;559:17-26. doi: 10.1016/bs.mie.2014.11.004. Epub 2015 Apr 15.

13.

Secretion and proteolysis of heterologous proteins fused to the Escherichia coli maltose binding protein in Pichia pastoris.

Li Z, Leung W, Yon A, Nguyen J, Perez VC, Vu J, Giang W, Luong LT, Phan T, Salazar KA, Gomez SR, Au C, Xiang F, Thomas DW, Franz AH, Lin-Cereghino J, Lin-Cereghino GP.

Protein Expr Purif. 2010 Jul;72(1):113-24. doi: 10.1016/j.pep.2010.03.004. Epub 2010 Mar 15.

14.

Phenylketonuria as a protein misfolding disease: The mutation pG46S in phenylalanine hydroxylase promotes self-association and fibril formation.

Leandro J, Simonsen N, Saraste J, Leandro P, Flatmark T.

Biochim Biophys Acta. 2011 Jan;1812(1):106-20. doi: 10.1016/j.bbadis.2010.09.015. Epub 2010 Oct 16.

15.

Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins.

Routzahn KM, Waugh DS.

J Struct Funct Genomics. 2002;2(2):83-92.

PMID:
12836665
16.

Unrelated solubility-enhancing fusion partners MBP and NusA utilize a similar mode of action.

Raran-Kurussi S, Waugh DS.

Biotechnol Bioeng. 2014 Dec;111(12):2407-11. doi: 10.1002/bit.25317. Epub 2014 Aug 25.

17.

Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.

Hewitt SN, Choi R, Kelley A, Crowther GJ, Napuli AJ, Van Voorhis WC.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1006-9. doi: 10.1107/S1744309111022159. Epub 2011 Aug 13.

18.

High-level soluble expression of bioactive porcine myostatin propeptide in E. coli.

Haq WY, Kang SK, Lee SB, Kang HC, Choi YJ, Lee CN, Kim YS.

Appl Microbiol Biotechnol. 2013 Oct;97(19):8517-27. doi: 10.1007/s00253-013-5134-0. Epub 2013 Aug 3.

PMID:
23912121
19.

Competitive interactions of ligands and macromolecular crowders with maltose binding protein.

Miklos AC, Sumpter M, Zhou HX.

PLoS One. 2013 Oct 4;8(10):e74969. doi: 10.1371/journal.pone.0074969. eCollection 2013.

20.

Kinetics and energetics of the translocation of maltose binding protein folding mutants.

Tomkiewicz D, Nouwen N, Driessen AJ.

J Mol Biol. 2008 Mar 14;377(1):83-90. doi: 10.1016/j.jmb.2008.01.014. Epub 2008 Jan 15.

PMID:
18241889
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