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Results: 1 to 20 of 177

1.

Cell-free metabolic engineering promotes high-level production of bioactive Gaussia princeps luciferase.

Goerke AR, Loening AM, Gambhir SS, Swartz JR.

Metab Eng. 2008 May-Jul;10(3-4):187-200. doi: 10.1016/j.ymben.2008.04.001. Epub 2008 May 2.

PMID:
18555198
[PubMed - indexed for MEDLINE]
2.

Biophysical characterization of highly active recombinant Gaussia luciferase expressed in Escherichia coli.

Rathnayaka T, Tawa M, Sohya S, Yohda M, Kuroda Y.

Biochim Biophys Acta. 2010 Sep;1804(9):1902-7. doi: 10.1016/j.bbapap.2010.04.014. Epub 2010 May 7.

PMID:
20452471
[PubMed - indexed for MEDLINE]
Free Article
3.

Solubilization and folding of a fully active recombinant Gaussia luciferase with native disulfide bonds by using a SEP-Tag.

Rathnayaka T, Tawa M, Nakamura T, Sohya S, Kuwajima K, Yohda M, Kuroda Y.

Biochim Biophys Acta. 2011 Dec;1814(12):1775-8. doi: 10.1016/j.bbapap.2011.09.001. Epub 2011 Sep 14.

PMID:
21945374
[PubMed - indexed for MEDLINE]
Free Article
4.

Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence.

Welsh JP, Patel KG, Manthiram K, Swartz JR.

Biochem Biophys Res Commun. 2009 Nov 27;389(4):563-8. doi: 10.1016/j.bbrc.2009.09.006.

PMID:
19825431
[PubMed - indexed for MEDLINE]
5.

Cell-free production of Gaussia princeps luciferase--antibody fragment bioconjugates for ex vivo detection of tumor cells.

Patel KG, Ng PP, Kuo CC, Levy S, Levy R, Swartz JR.

Biochem Biophys Res Commun. 2009 Dec 18;390(3):971-6. doi: 10.1016/j.bbrc.2009.10.087. Epub 2009 Oct 21.

PMID:
19852937
[PubMed - indexed for MEDLINE]
6.

The level of synthesis and secretion of Gaussia princeps luciferase in transfected CHO cells is heavily dependent on the choice of signal peptide.

Knappskog S, Ravneberg H, Gjerdrum C, Trösse C, Stern B, Pryme IF.

J Biotechnol. 2007 Mar 10;128(4):705-15. Epub 2006 Dec 12.

PMID:
17316861
[PubMed - indexed for MEDLINE]
7.

Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization.

Verhaegent M, Christopoulos TK.

Anal Chem. 2002 Sep 1;74(17):4378-85.

PMID:
12236345
[PubMed - indexed for MEDLINE]
8.

Cell-free production of aggregation-prone proteins in soluble and active forms.

Kang SH, Kim DM, Kim HJ, Jun SY, Lee KY, Kim HJ.

Biotechnol Prog. 2005 Sep-Oct;21(5):1412-9.

PMID:
16209544
[PubMed - indexed for MEDLINE]
9.

Gaussia-luciferase as a sensitive reporter gene for monitoring promoter activity in the nucleus of the green alga Chlamydomonas reinhardtii.

Ruecker O, Zillner K, Groebner-Ferreira R, Heitzer M.

Mol Genet Genomics. 2008 Aug;280(2):153-62. doi: 10.1007/s00438-008-0352-3. Epub 2008 May 31.

PMID:
18516621
[PubMed - indexed for MEDLINE]
10.

Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo.

Tannous BA, Kim DE, Fernandez JL, Weissleder R, Breakefield XO.

Mol Ther. 2005 Mar;11(3):435-43.

PMID:
15727940
[PubMed - indexed for MEDLINE]
11.

Identification of two catalytic domains in a luciferase secreted by the copepod Gaussia princeps.

Inouye S, Sahara Y.

Biochem Biophys Res Commun. 2008 Jan 4;365(1):96-101. Epub 2007 Nov 5.

PMID:
17981153
[PubMed - indexed for MEDLINE]
12.

Identification of cellular genes critical to recombinant protein production using a Gaussia luciferase-based siRNA screening system.

Lwa TR, Tan CH, Lew QJ, Chu KL, Tan J, Lee YY, Chao SH.

J Biotechnol. 2010 Apr 15;146(4):160-8. doi: 10.1016/j.jbiotec.2010.02.016. Epub 2010 Feb 25.

PMID:
20188772
[PubMed - indexed for MEDLINE]
13.

Recombinant Metridia luciferase isoforms: expression, refolding and applicability for in vitro assay.

Borisova VV, Frank LA, Markova SV, Burakova LP, Vysotski ES.

Photochem Photobiol Sci. 2008 Sep;7(9):1025-31. doi: 10.1039/b807271j. Epub 2008 Jun 17.

PMID:
18754048
[PubMed - indexed for MEDLINE]
14.

Amino acid stabilization for cell-free protein synthesis by modification of the Escherichia coli genome.

Michel-Reydellet N, Calhoun K, Swartz J.

Metab Eng. 2004 Jul;6(3):197-203.

PMID:
15256209
[PubMed - indexed for MEDLINE]
15.

Gaussia princeps luciferase as a reporter for transcriptional activity, protein secretion, and protein-protein interactions in Salmonella enterica serovar typhimurium.

Wille T, Blank K, Schmidt C, Vogt V, Gerlach RG.

Appl Environ Microbiol. 2012 Jan;78(1):250-7. doi: 10.1128/AEM.06670-11. Epub 2011 Oct 21.

PMID:
22020521
[PubMed - indexed for MEDLINE]
Free PMC Article
16.

Expression of active murine granulocyte-macrophage colony-stimulating factor in an Escherichia coli cell-free system.

Yang J, Kanter G, Voloshin A, Levy R, Swartz JR.

Biotechnol Prog. 2004 Nov-Dec;20(6):1689-96.

PMID:
15575700
[PubMed - indexed for MEDLINE]
17.

Total amino acid stabilization during cell-free protein synthesis reactions.

Calhoun KA, Swartz JR.

J Biotechnol. 2006 May 17;123(2):193-203. Epub 2006 Jan 26.

PMID:
16442654
[PubMed - indexed for MEDLINE]
18.

Fusion of Gaussia luciferase to an engineered anti-carcinoembryonic antigen (CEA) antibody for in vivo optical imaging.

Venisnik KM, Olafsen T, Gambhir SS, Wu AM.

Mol Imaging Biol. 2007 Sep-Oct;9(5):267-77.

PMID:
17577599
[PubMed - indexed for MEDLINE]
19.

Development of cell-free protein synthesis platforms for disulfide bonded proteins.

Goerke AR, Swartz JR.

Biotechnol Bioeng. 2008 Feb 1;99(2):351-67.

PMID:
17626291
[PubMed - indexed for MEDLINE]
20.

A highly sensitive protein-protein interaction assay based on Gaussia luciferase.

Remy I, Michnick SW.

Nat Methods. 2006 Dec;3(12):977-9. Epub 2006 Nov 12.

PMID:
17099704
[PubMed - indexed for MEDLINE]

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