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Results: 1 to 20 of 321

1.

A novel strategy for generation of monoclonal antibodies from single B cells using rt-PCR technique and in vitro expression.

Jiang X, Suzuki H, Hanai Y, Wada F, Hitomi K, Yamane T, Nakano H.

Biotechnol Prog. 2006 Jul-Aug;22(4):979-88.

PMID:
16889373
[PubMed - indexed for MEDLINE]
2.

In vitro generation of anti-hepatitis B monoclonal antibodies from a single plasma cell using single-cell RT-PCR and cell-free protein synthesis.

Sabrina Y, Ali M, Nakano H.

J Biosci Bioeng. 2010 Jan;109(1):75-82. doi: 10.1016/j.jbiosc.2009.07.001. Epub 2009 Jul 28.

PMID:
20129087
[PubMed - indexed for MEDLINE]
3.

Antibody generation through B cell panning on antigen followed by in situ culture and direct RT-PCR on cells harvested en masse from antigen-positive wells.

Lightwood DJ, Carrington B, Henry AJ, McKnight AJ, Crook K, Cromie K, Lawson AD.

J Immunol Methods. 2006 Oct 20;316(1-2):133-43. Epub 2006 Sep 18.

PMID:
17027850
[PubMed - indexed for MEDLINE]
4.

Cloning and expression in E. coli of a functional Fab fragment obtained from single human lymphocyte against anthrax toxin.

Masri SA, Rast H, Hu WG, Nagata LP, Chau D, Jager S, Mah D.

Mol Immunol. 2007 Mar;44(8):2101-6. Epub 2006 Oct 12.

PMID:
17045651
[PubMed - indexed for MEDLINE]
5.

Selection of a human anti-progesterone antibody fragment from a transgenic mouse library by ARM ribosome display.

He M, Menges M, Groves MA, Corps E, Liu H, Brüggemann M, Taussig MJ.

J Immunol Methods. 1999 Dec 10;231(1-2):105-17.

PMID:
10648931
[PubMed - indexed for MEDLINE]
6.

Two-phase approach for the expression of high-affinity human anti-human immunodeficiency virus immunoglobulin Fab domains in Escherichia coli.

Takeda S, Dorfman NA, Robert-Guroff M, Notkins AL, Rando RF.

Hybridoma. 1995 Feb;14(1):9-18.

PMID:
7768538
[PubMed - indexed for MEDLINE]
7.

Cloning and expression of murine Ig genes from single B cells.

Tiller T, Busse CE, Wardemann H.

J Immunol Methods. 2009 Oct 31;350(1-2):183-93. doi: 10.1016/j.jim.2009.08.009. Epub 2009 Aug 27.

PMID:
19716372
[PubMed - indexed for MEDLINE]
8.

Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles.

Weitkamp JH, Kallewaard N, Kusuhara K, Feigelstock D, Feng N, Greenberg HB, Crowe JE Jr.

J Immunol Methods. 2003 Apr 1;275(1-2):223-37.

PMID:
12667686
[PubMed - indexed for MEDLINE]
9.

A recombinant human Fab expressed in Escherichia coli neutralizes rabies virus.

Cheung SC, Dietzschold B, Koprowski H, Notkins AL, Rando RF.

J Virol. 1992 Nov;66(11):6714-20.

PMID:
1404611
[PubMed - indexed for MEDLINE]
Free PMC Article
10.

Both VH and VL chains of polyreactive IgM antibody are required for polyreactivity: expression of Fab in Escherichia coli.

Cheung SC, Takeda S, Notkins AL.

Clin Exp Immunol. 1995 Aug;101(2):383-6.

PMID:
7648724
[PubMed - indexed for MEDLINE]
Free PMC Article
11.

Cloning and characterization of 1116NS19.9 heavy and light chain cDNAs and expression of antibody fragments in Escherichia coli.

Tonge DW, Hennam JF, Greene AR, Lee ID, Edge MD.

Year Immunol. 1993;7:56-62. No abstract available.

PMID:
8372513
[PubMed - indexed for MEDLINE]
12.

Generation and selection of immunized Fab phage display library against human B cell lymphoma.

Shen Y, Yang X, Dong N, Xie X, Bai X, Shi Y.

Cell Res. 2007 Jul;17(7):650-60.

PMID:
17621306
[PubMed - indexed for MEDLINE]
Free Article
13.

[Generation, screening and preliminary identification of specific Fab phage antibody library against Daudi cell strain].

Shen YM, Yang XC, Dong NZ, Bai X.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009 Feb;25(2):150-4. Chinese.

PMID:
19174013
[PubMed - indexed for MEDLINE]
14.

Functional humanization of an anti-CD30 Fab fragment for the immunotherapy of Hodgkin's lymphoma using an in vitro evolution approach.

Schlapschy M, Gruber H, Gresch O, Schäfer C, Renner C, Pfreundschuh M, Skerra A.

Protein Eng Des Sel. 2004 Dec;17(12):847-60. Epub 2005 Feb 11.

PMID:
15708864
[PubMed - indexed for MEDLINE]
Free Article
15.

A novel strategy for generating monoclonal antibodies from single, isolated lymphocytes producing antibodies of defined specificities.

Babcook JS, Leslie KB, Olsen OA, Salmon RA, Schrader JW.

Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7843-8.

PMID:
8755564
[PubMed - indexed for MEDLINE]
Free PMC Article
16.

[Construction and expression of single chain Fv antibody against human bladder carcinoma].

Zhang M, Yu L, Huang H.

Zhonghua Wai Ke Za Zhi. 2001 Oct;39(10):792-5. Chinese.

PMID:
16201198
[PubMed - indexed for MEDLINE]
17.

Production of recombinant Ig molecules from antigen-selected single B cells and restricted usage of Ig-gene segments by anti-D antibodies.

Dohmen SE, Mulder A, Verhagen OJ, Eijsink C, Franke-van Dijk ME, van der Schoot CE.

J Immunol Methods. 2005 Mar;298(1-2):9-20.

PMID:
15847793
[PubMed - indexed for MEDLINE]
18.

Molecular basis for the interaction of the hepatitis B virus core antigen with the surface immunoglobulin receptor on naive B cells.

Lazdina U, Cao T, Steinbergs J, Alheim M, Pumpens P, Peterson DL, Milich DR, Leroux-Roels G, Sällberg M.

J Virol. 2001 Jul;75(14):6367-74.

PMID:
11413303
[PubMed - indexed for MEDLINE]
Free PMC Article
19.

High affinity single-chain Fv antibody fragments protecting the human nicotinic acetylcholine receptor.

Tsantili P, Tzartos SJ, Mamalaki A.

J Neuroimmunol. 1999 Feb 1;94(1-2):15-27.

PMID:
10376932
[PubMed - indexed for MEDLINE]
20.

Bacterial expression of a neutralizing mouse monoclonal antibody Fab fragment to a 150-kilodalton surface antigen of Entamoeba histolytica.

Tachibana H, Takekoshi M, Cheng XJ, Maeda F, Aotsuka S, Ihara S.

Am J Trop Med Hyg. 1999 Jan;60(1):35-40.

PMID:
9988319
[PubMed - indexed for MEDLINE]
Free Article
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