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Results: 1 to 20 of 314

1.

Standardization of cytokine flow cytometry assays.

Maecker HT, Rinfret A, D'Souza P, Darden J, Roig E, Landry C, Hayes P, Birungi J, Anzala O, Garcia M, Harari A, Frank I, Baydo R, Baker M, Holbrook J, Ottinger J, Lamoreaux L, Epling CL, Sinclair E, Suni MA, Punt K, Calarota S, El-Bahi S, Alter G, Maila H, Kuta E, Cox J, Gray C, Altfeld M, Nougarede N, Boyer J, Tussey L, Tobery T, Bredt B, Roederer M, Koup R, Maino VC, Weinhold K, Pantaleo G, Gilmour J, Horton H, Sekaly RP.

BMC Immunol. 2005 Jun 24;6:13.

PMID:
15978127
[PubMed - indexed for MEDLINE]
Free PMC Article
2.

Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT.

Maecker HT, Moon J, Bhatia S, Ghanekar SA, Maino VC, Payne JK, Kuus-Reichel K, Chang JC, Summers A, Clay TM, Morse MA, Lyerly HK, DeLaRosa C, Ankerst DP, Disis ML.

BMC Immunol. 2005 Jul 18;6:17.

PMID:
16026627
[PubMed - indexed for MEDLINE]
Free PMC Article
3.

Precision and linearity targets for validation of an IFNgamma ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides.

Maecker HT, Hassler J, Payne JK, Summers A, Comatas K, Ghanayem M, Morse MA, Clay TM, Lyerly HK, Bhatia S, Ghanekar SA, Maino VC, Delarosa C, Disis ML.

BMC Immunol. 2008 Mar 17;9:9. doi: 10.1186/1471-2172-9-9.

PMID:
18366814
[PubMed - indexed for MEDLINE]
Free PMC Article
4.

VACUTAINER CPT and Ficoll density gradient separation perform equivalently in maintaining the quality and function of PBMC from HIV seropositive blood samples.

Ruitenberg JJ, Mulder CB, Maino VC, Landay AL, Ghanekar SA.

BMC Immunol. 2006 May 25;7:11.

PMID:
16725038
[PubMed - indexed for MEDLINE]
Free PMC Article
5.

Use of overlapping peptide mixtures as antigens for cytokine flow cytometry.

Maecker HT, Dunn HS, Suni MA, Khatamzas E, Pitcher CJ, Bunde T, Persaud N, Trigona W, Fu TM, Sinclair E, Bredt BM, McCune JM, Maino VC, Kern F, Picker LJ.

J Immunol Methods. 2001 Sep 1;255(1-2):27-40.

PMID:
11470284
[PubMed - indexed for MEDLINE]
6.

ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring--even in hands of ELISPOT-inexperienced investigators.

Zhang W, Caspell R, Karulin AY, Ahmad M, Haicheur N, Abdelsalam A, Johannesen K, Vignard V, Dudzik P, Georgakopoulou K, Mihaylova A, Silina K, Aptsiauri N, Adams V, Lehmann PV, McArdle S.

J Immunotoxicol. 2009 Dec;6(4):227-34. doi: 10.3109/15476910903317546.

PMID:
19908941
[PubMed - indexed for MEDLINE]
7.

Performance of plate-based cytokine flow cytometry with automated data analysis.

Suni MA, Dunn HS, Orr PL, de Laat R, Sinclair E, Ghanekar SA, Bredt BM, Dunne JF, Maino VC, Maecker HT.

BMC Immunol. 2003 Sep 2;4:9. Epub 2003 Sep 2.

PMID:
12952557
[PubMed - indexed for MEDLINE]
Free PMC Article
8.

Enumerating antigen-specific T-cell responses in peripheral blood: a comparison of peptide MHC Tetramer, ELISpot, and intracellular cytokine analysis.

Hobeika AC, Morse MA, Osada T, Ghanayem M, Niedzwiecki D, Barrier R, Lyerly HK, Clay TM.

J Immunother. 2005 Jan-Feb;28(1):63-72.

PMID:
15614046
[PubMed - indexed for MEDLINE]
9.

Standardization and optimization of multiparameter intracellular cytokine staining.

Nomura L, Maino VC, Maecker HT.

Cytometry A. 2008 Nov;73(11):984-91. doi: 10.1002/cyto.a.20602.

PMID:
18612990
[PubMed - indexed for MEDLINE]
Free Article
10.

Quality assurance of intracellular cytokine staining assays: analysis of multiple rounds of proficiency testing.

Jaimes MC, Maecker HT, Yan M, Maino VC, Hanley MB, Greer A, Darden JM, D'Souza MP.

J Immunol Methods. 2011 Jan 5;363(2):143-57. doi: 10.1016/j.jim.2010.08.004. Epub 2010 Aug 19.

PMID:
20727897
[PubMed - indexed for MEDLINE]
Free PMC Article
11.

CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.

Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M.

J Immunol Methods. 2003 Jul;278(1-2):79-93.

PMID:
12957398
[PubMed - indexed for MEDLINE]
12.

Optimization of whole blood antigen-specific cytokine assays for CD4(+) T cells.

Nomura LE, Walker JM, Maecker HT.

Cytometry. 2000 May 1;40(1):60-8.

PMID:
10754518
[PubMed - indexed for MEDLINE]
13.

Flow cytometric determination of intracellular or secreted IFNgamma for the quantification of antigen reactive T cells.

Asemissen AM, Nagorsen D, Keilholz U, Letsch A, Schmittel A, Thiel E, Scheibenbogen C.

J Immunol Methods. 2001 May 1;251(1-2):101-8.

PMID:
11292486
[PubMed - indexed for MEDLINE]
14.

Optimum culture conditions for specific and nonspecific activation of whole blood and PBMC for intracellular cytokine assessment by flow cytometry.

Godoy-Ramirez K, Franck K, Mahdavifar S, Andersson L, Gaines H.

J Immunol Methods. 2004 Sep;292(1-2):1-15.

PMID:
15350507
[PubMed - indexed for MEDLINE]
15.

Enumeration of antigen-specific CD8+ T lymphocytes by single-platform, HLA tetramer-based flow cytometry: a European multicenter evaluation.

Heijnen IA, Barnett D, Arroz MJ, Barry SM, Bonneville M, Brando B, D'hautcourt JL, Kern F, Tötterman TH, Marijt EW, Bossy D, Preijers FW, Rothe G, Gratama JW; European Working Group on Clinical Cell Analysis.

Cytometry B Clin Cytom. 2004 Nov;62(1):1-13.

PMID:
15468327
[PubMed - indexed for MEDLINE]
Free Article
16.

Antigen-specific T cell responses induced by Towne cytomegalovirus (CMV) vaccine in CMV-seronegative vaccine recipients.

Jacobson MA, Sinclair E, Bredt B, Agrillo L, Black D, Epling CL, Carvidi A, Ho T, Bains R, Adler SP.

J Clin Virol. 2006 Mar;35(3):332-7. Epub 2006 Jan 18.

PMID:
16387547
[PubMed - indexed for MEDLINE]
17.

Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry.

Suni MA, Picker LJ, Maino VC.

J Immunol Methods. 1998 Mar 1;212(1):89-98.

PMID:
9671156
[PubMed - indexed for MEDLINE]
18.

Flow cytometric lymphocyte subset enumeration: 10 years of external quality assessment in the Benelux countries.

Levering WH, van Wieringen WN, Kraan J, van Beers WA, Sintnicolaas K, van Rhenen DJ, Gratama JW.

Cytometry B Clin Cytom. 2008 Mar;74(2):79-90.

PMID:
17849485
[PubMed - indexed for MEDLINE]
Free Article
19.

Standardization and performance evaluation of mononuclear cell cytokine secretion assays in a multicenter study.

Shreffler WG, Visness CM, Burger M, Cruikshank WW, Lederman HM, de la Morena M, Grindle K, Calatroni A, Sampson HA, Gern JE.

BMC Immunol. 2006 Dec 12;7:29.

PMID:
17156490
[PubMed - indexed for MEDLINE]
Free PMC Article
20.

Analytical performance of a standardized single-platform MHC tetramer assay for the identification and enumeration of CMV-specific CD8+ T lymphocytes.

Brooimans RA, Boyce CS, Popma J, Broyles DA, Gratama JW, Southwick PC, Keeney M.

Cytometry A. 2008 Nov;73(11):992-1000. doi: 10.1002/cyto.a.20641.

PMID:
18792106
[PubMed - indexed for MEDLINE]
Free Article

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