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Appl Environ Microbiol. 1998 Jul;64(7):2710-5.

Plasposons: modular self-cloning minitransposon derivatives for rapid genetic analysis of gram-negative bacterial genomes.

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  • 1Biotechnology Center for Agriculture, Cook College, Rutgers University, New Brunswick, New Jersey 08901-8520, USA.

Abstract

A series of modular mini-transposon derivatives which permit the rapid cloning and mapping of the DNA flanking the minitransposon's site of insertion has been developed. The basic plasposon, named TnMod, consists of the Tn5 inverted repeats, a conditional origin of replication, rare restriction endonuclease multiple cloning sites, and exchangeable antibiotic resistance cassettes. The broad host range and low target DNA sequence specificity of the Tn5 transposase, in combination with the flexibility afforded by the modular arrangement of TnMod, result in a versatile tool for the mapping of insertional mutations and the rapid recovery of clones from gram-negative bacteria.

PMID:
9647854
PMCID:
PMC106450
[PubMed - indexed for MEDLINE]
Free PMC Article
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