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Gene. 1993 Feb 14;124(1):83-5.

The pRSET family of T7 promoter expression vectors for Escherichia coli.

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  • Salk Institute, San Diego, CA 92186-5800.


A family of eight T7 promoter-based expression plasmids is presented. These are high-copy-number vectors featuring translational start and stop elements and a multiple cloning site (polylinker) with eleven unique restriction sites in all six reading frames. Depending on the cloning strategy used, recombinant proteins may contain either short vector-encoded fusion fragments or no fusion fragments at all. Following promoter induction, proteins are usually produced at a high level.

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