Abstract
A survey to measure transmitted HIV-1 drug resistance (DR) was conducted in 2006 following the World Health Organization threshold survey protocol. Dried blood spots (DBS) were prepared from 53 newly HIV-1-diagnosed patients. Protease and reverse transcriptase (RT) gene regions were sequenced using a broadly sensitive genotyping assay and analyses to identify DR mutations and determine phylogeny of the HIV-1 strains were conducted. Forty-six of the 47 successfully genotyped DBS had no transmitted DR mutations; one had an NNRTI mutation (K101E) in the RT region. Phylogenetic analyses revealed 21 (44.7%) were CRF01_AE, 9 (19.1%) B, 6 (12.8%) CRF07_BC, 3 (6.4%) each of CRF08_BC and C, and 2 (4.3%) B/C unique recombinant forms (URF). The remaining three were one each of A/B, A/C, and unclassifiable. Our analyses indicate that the prevalence of transmitted DR in this population is low and the HIV-1 epidemic in the area was characterized by multiple subtypes and recombinant forms.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adolescent
-
China
-
Cluster Analysis
-
Drug Resistance, Viral*
-
Female
-
Genotype
-
HIV Infections / transmission*
-
HIV Infections / virology*
-
HIV Protease / genetics
-
HIV Reverse Transcriptase / genetics
-
HIV-1 / classification
-
HIV-1 / drug effects*
-
HIV-1 / genetics
-
HIV-1 / isolation & purification
-
Humans
-
Male
-
Molecular Sequence Data
-
Phylogeny
-
Recombination, Genetic
-
Sequence Analysis, DNA
-
Sequence Homology
-
Young Adult
Substances
-
reverse transcriptase, Human immunodeficiency virus 1
-
HIV Reverse Transcriptase
-
HIV Protease
Associated data
-
GENBANK/GQ443552
-
GENBANK/GQ443553
-
GENBANK/GQ443554
-
GENBANK/GQ443555
-
GENBANK/GQ443556
-
GENBANK/GQ443557
-
GENBANK/GQ443558
-
GENBANK/GQ443559
-
GENBANK/GQ443560
-
GENBANK/GQ443561
-
GENBANK/GQ443562
-
GENBANK/GQ443563
-
GENBANK/GQ443564
-
GENBANK/GQ443565
-
GENBANK/GQ443566
-
GENBANK/GQ443567
-
GENBANK/GQ443568
-
GENBANK/GQ443569
-
GENBANK/GQ443570
-
GENBANK/GQ443571
-
GENBANK/GQ443572
-
GENBANK/GQ443573
-
GENBANK/GQ443574
-
GENBANK/GQ443575
-
GENBANK/GQ443576
-
GENBANK/GQ443577
-
GENBANK/GQ443578
-
GENBANK/GQ443579
-
GENBANK/GQ443580
-
GENBANK/GQ443581
-
GENBANK/GQ443582
-
GENBANK/GQ443583
-
GENBANK/GQ443584
-
GENBANK/GQ443585
-
GENBANK/GQ443586
-
GENBANK/GQ443587
-
GENBANK/GQ443588
-
GENBANK/GQ443589
-
GENBANK/GQ443590
-
GENBANK/GQ443591
-
GENBANK/GQ443592
-
GENBANK/GQ443593
-
GENBANK/GQ443594
-
GENBANK/GQ443595
-
GENBANK/GQ443596
-
GENBANK/GQ443597
-
GENBANK/GQ443598