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112 results

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Page 1
Steady-State and Time-Resolved Fluorescence Study of Selected Tryptophan-Containing Peptides in an AOT Reverse Micelle Environment.
Gałęcki K, Kowalska-Baron A, Nowak KE, Gajda A, Kolesińska B. Gałęcki K, et al. Int J Mol Sci. 2023 Oct 22;24(20):15438. doi: 10.3390/ijms242015438. Int J Mol Sci. 2023. PMID: 37895121 Free PMC article.
The aim of this study was to demonstrate the utility of time-resolved fluorescence spectroscopy in the detection of subtle changes in the local microenvironment of a tryptophan chromophore in a confined and crowded medium of AOT reverse micelles, which …
The aim of this study was to demonstrate the utility of time-resolved fluorescence spectroscopy in the detection …
Polar plot representation of time-resolved fluorescence.
Eichorst JP, Wen Teng K, Clegg RM. Eichorst JP, et al. Methods Mol Biol. 2014;1076:97-112. doi: 10.1007/978-1-62703-649-8_6. Methods Mol Biol. 2014. PMID: 24108625
Measuring changes in a molecule's fluorescence emission is a common technique to study complex biological systems such as cells and tissues. Although the steady-state fluorescence intensity is frequently used, measuring the average …
Measuring changes in a molecule's fluorescence emission is a common technique to study complex biological system …
Photophysical and Protonation Time Resolved Studies of Donor-Acceptor Branched Systems With Pyridine Acceptors.
Kournoutas F, Seintis K, Karakostas N, Tydlitát J, Achelle S, Pistolis G, Bureš F, Fakis M. Kournoutas F, et al. J Phys Chem A. 2019 Jan 17;123(2):417-428. doi: 10.1021/acs.jpca.8b08628. Epub 2019 Jan 7. J Phys Chem A. 2019. PMID: 30365895 Free article.
A comparative study of the photophysical properties of octupolar pyridyl-terminated triphenylamine molecule, with its quadrupolar and dipolar analogues, by means of ambient and low temperature steady state spectroscopy and femtosecond to nanosecond …
A comparative study of the photophysical properties of octupolar pyridyl-terminated triphenylamine molecule, with its quadrupo …
Use of Time-Resolved Fluorescence to Monitor Bioactive Compounds in Plant Based Foodstuffs.
Lemos MA, Sárniková K, Bot F, Anese M, Hungerford G. Lemos MA, et al. Biosensors (Basel). 2015 Jun 26;5(3):367-97. doi: 10.3390/bios5030367. Biosensors (Basel). 2015. PMID: 26132136 Free PMC article. Review.
Time-resolved fluorescence techniques have recently come to the fore, as they offer the ability to obtain more information, coupled with the fact that the fluorescence lifetime is an absolute measure, while steady state just provides rela
Time-resolved fluorescence techniques have recently come to the fore, as they offer the ability to obtain more informat
Selective steady-state and time-resolved fluorescence spectroscopy of an HLA-A2-peptide complex.
Gakamsky DM, Haas E, Robbins P, Strominger JL, Pecht I. Gakamsky DM, et al. Immunol Lett. 1995 Jan;44(2-3):195-201. doi: 10.1016/0165-2478(94)00214-c. Immunol Lett. 1995. PMID: 7797251
The human class I major histocompatibility complex (MHC) encoded molecule HLA-A2 loaded with the high-affinity peptide GILGRVFTL (p790), was studied by means of steady-state and picosecond fluorescence intensity and fluorescence anisotropy metho …
The human class I major histocompatibility complex (MHC) encoded molecule HLA-A2 loaded with the high-affinity peptide GILGRVFTL (p79 …
Effects of solvent and micellar encapsulation on the photostability of avobenzone.
Hanson KM, Cutuli M, Rivas T, Antuna M, Saoub J, Tierce NT, Bardeen CJ. Hanson KM, et al. Photochem Photobiol Sci. 2020 Mar 1;19(3):390-398. doi: 10.1039/c9pp00483a. Epub 2020 Feb 26. Photochem Photobiol Sci. 2020. PMID: 32100782
AV is highly susceptible to photoinduced diketonization in both nonpolar solvents and in aqueous aggregates but is considerably more stable in polar, protic solvents like methanol. By studying its stability in different surfactant solutions, we show that incorporati …
AV is highly susceptible to photoinduced diketonization in both nonpolar solvents and in aqueous aggregates but is considerably more stable …
Spectroscopic studies on human serum albumin and methemalbumin: optical, steady-state, and picosecond time-resolved fluorescence studies, and kinetics of substrate oxidation by methemalbumin.
Kamal JK, Behere DV. Kamal JK, et al. J Biol Inorg Chem. 2002 Mar;7(3):273-83. doi: 10.1007/s007750100294. Epub 2001 Oct 2. J Biol Inorg Chem. 2002. PMID: 11935351
Decay associated spectra (DAS) indicate that the observed lifetime variation with wavelength is predominantly due to ground state heterogeneity, though solvent dipolar relaxation also contributes. Time-resolved fluorescence anisotropy measurements of the Trp2 …
Decay associated spectra (DAS) indicate that the observed lifetime variation with wavelength is predominantly due to ground state heterogene …
Unraveling the Interaction of Diflunisal with Cyclodextrin and Lysozyme by Fluorescence Spectroscopy.
Agarwala P, Ghosh A, Hazarika P, Acharjee D, Ghosh S, Rout D, Sasmal DK. Agarwala P, et al. J Phys Chem B. 2023 Nov 16;127(45):9710-9723. doi: 10.1021/acs.jpcb.3c04295. Epub 2023 Nov 2. J Phys Chem B. 2023. PMID: 37917720
Here, we present our findings on the interaction of the anti-inflammatory drug diflunisal with the drug carrier cyclodextrin (CD) and the protein lysozyme, utilizing steady-state and time-resolved fluorescence spectroscopy. Our findings reveal a …
Here, we present our findings on the interaction of the anti-inflammatory drug diflunisal with the drug carrier cyclodextrin (CD) and the pr …
Fluorescence Quenching Studies on the Interactions between Chosen Fluoroquinolones and Selected Stable TEMPO and PROXYL Nitroxides.
Żamojć K, Bylińska I, Wiczk W, Chmurzyński L. Żamojć K, et al. Int J Mol Sci. 2021 Jan 17;22(2):885. doi: 10.3390/ijms22020885. Int J Mol Sci. 2021. PMID: 33477329 Free PMC article.
The influence of the stable 2,2,6,6-tetramethylpiperidinyl-N-oxyl (TEMPO) nitroxide and its six C4-substituted derivatives, as well as two C3-substituted analogues of 2,2,5,5-tetramethylpyrrolidynyl-N-oxyl (PROXYL) nitroxide on the chosen fluoroquinolone antibiotics (marbofloxaci …
The influence of the stable 2,2,6,6-tetramethylpiperidinyl-N-oxyl (TEMPO) nitroxide and its six C4-substituted derivatives, as well as two C …
A general approach for detecting folding intermediates from steady-state and time-resolved fluorescence of single-tryptophan-containing proteins.
Laptenok SP, Visser NV, Engel R, Westphal AH, van Hoek A, van Mierlo CP, van Stokkum IH, van Amerongen H, Visser AJ. Laptenok SP, et al. Biochemistry. 2011 May 3;50(17):3441-50. doi: 10.1021/bi101965d. Epub 2011 Apr 7. Biochemistry. 2011. PMID: 21425856
During denaturant-induced equilibrium (un)folding of wild-type apoflavodoxin from Azotobacter vinelandii, a molten globule-like folding intermediate is formed. This wild-type protein contains three tryptophans. In this study, we use a general approach to analyze time
During denaturant-induced equilibrium (un)folding of wild-type apoflavodoxin from Azotobacter vinelandii, a molten globule-like folding inte …
112 results