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Determination of rat serum esterase activities by an HPLC method using S-acetylthiocholine iodide and p-nitrophenyl acetate.
Anal Biochem. 2008 Oct 1;381(1):113-22. doi: 10.1016/j.ab.2008.06.019. Epub 2008 Jun 18.
Anal Biochem. 2008.
PMID: 18602882
We have developed a high-performance liquid chromatography (HPLC) assay for the determination of S-acetylthiocholine (ATC) and p-nitrophenyl acetate (NPA) hydrolyzing activities of rat serum esterases based on ion pair chromatography with on-line radiochemical and u …
We have developed a high-performance liquid chromatography (HPLC) assay for the determination of S-acetylthiocholine (ATC) and …
Enzyme colorimetric cellulose membrane bioactivity strips based on acetylcholinesterase immobilization for inhibitors preliminary screening.
Chen Y, Zhang X, Luo X.
Chen Y, et al.
Colloids Surf B Biointerfaces. 2023 Mar;223:113184. doi: 10.1016/j.colsurfb.2023.113184. Epub 2023 Jan 31.
Colloids Surf B Biointerfaces. 2023.
PMID: 36739673
When the enzyme activity of AChE was inhibited after incubation with inhibitors, the hydrolysis of S-Acetylthiocholine iodide decreased, consequently, the 5-thio-2-nitrobenzoic acid generated by the reaction with 5,5'-dithiobis (2-nitrobenzoic acid) also decr …
When the enzyme activity of AChE was inhibited after incubation with inhibitors, the hydrolysis of S-Acetylthiocholine iodi …
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Selective and sensitive detection of acetylcholinesterase activity using denatured protein-protected gold nanoclusters as a label-free probe.
Li H, Guo Y, Xiao L, Chen B.
Li H, et al.
Analyst. 2014 Jan 7;139(1):285-9. doi: 10.1039/c3an01736b. Epub 2013 Nov 19.
Analyst. 2014.
PMID: 24251311
The fluorescence of the AuNCs was quenched by thiocholine that was produced from the AChE hydrolysis of S-acetylthiocholine iodide (ACTI) to detect the AChE activity. ...
The fluorescence of the AuNCs was quenched by thiocholine that was produced from the AChE hydrolysis of S-acetylthiocholine …
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A small-volume bioassay for quantification of the esterase inhibiting potency of mixtures of organophosphate and carbamate insecticides in rainwater: development and optimization.
Hamers T, Molin KR, Koeman JH, Murk AJ.
Hamers T, et al.
Toxicol Sci. 2000 Nov;58(1):60-7. doi: 10.1093/toxsci/58.1.60.
Toxicol Sci. 2000.
PMID: 11053541
Purified acetylcholinesterase (AChE) from electric eel (Electrophorus electricus) and carboxylesterases from a homogenate of honeybee heads (Apis mellifera) were used as esterases, each having different affinities for the substrates S-acetylthiocholine-iodide …
Purified acetylcholinesterase (AChE) from electric eel (Electrophorus electricus) and carboxylesterases from a homogenate of honeybee heads …
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