Blinking triggered by the change in the solvent accessibility of a fluorescent molecule

Chem Commun (Camb). 2014 Sep 18;50(72):10478-81. doi: 10.1039/c4cc00377b.

Abstract

The more a fluorescent molecule is exposed to a solvent, the faster its triplet excited state is quenched by molecular oxygen. The changes in the solvent accessibility of a fluorescent molecule were probed by measuring the duration of the off time during the blinking of the fluorescence, which enabled analysis of the function of a molecular beacon-type probe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / chemistry
  • Fluorescent Dyes / chemistry*
  • Methylene Blue / chemistry
  • Oxygen / chemistry
  • Quantum Theory
  • Rhodamines / chemistry
  • Solvents / chemistry*
  • Spectrometry, Fluorescence

Substances

  • Fluorescent Dyes
  • Rhodamines
  • Solvents
  • rhodamine 6G
  • DNA
  • Oxygen
  • Methylene Blue