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One-Pot Generating Subunit Vaccine with High Encapsulating Efficiency and Fast Lysosome Escape for Potent Cellular Immune Response.
Hao P, Wu X, Wang L, Wei S, Xu H, Huang W, Li Y, Zhang T, Zan X. Hao P, et al. Bioconjug Chem. 2020 Aug 19;31(8):1917-1927. doi: 10.1021/acs.bioconjchem.0c00274. Epub 2020 Jul 17. Bioconjug Chem. 2020. PMID: 32639141
To address these issues, here, for the first time, we developed a one-pot method to produce a subunit vaccine by using hexa-histidine metal assembly (HmA) to codeliver tumor-associated antigens (GP100, a peptide KTWGQYWQV) and adjuvant (CpG). The generation of subunit vacc …
To address these issues, here, for the first time, we developed a one-pot method to produce a subunit vaccine by using hexa-histidine metal …
Mass-spectrometric evaluation of HLA-A*0201-associated peptides identifies dominant naturally processed forms of CTL epitopes from MART-1 and gp100.
Skipper JC, Gulden PH, Hendrickson RC, Harthun N, Caldwell JA, Shabanowitz J, Engelhard VH, Hunt DF, Slingluff CL Jr. Skipper JC, et al. Int J Cancer. 1999 Aug 27;82(5):669-77. doi: 10.1002/(sici)1097-0215(19990827)82:5<669::aid-ijc9>3.0.co;2-#. Int J Cancer. 1999. PMID: 10417764 Free article.
To that end, we have isolated peptides directly from HLA-A*0201 molecules of human melanoma cells and have determined that naturally processed epitopes for HLA-A*0201-restricted, melanoma-reactive CTLs include the nonamers MART-1(27-35) (AAGIGILTV), gp100(154-162) (KTWGQYWQV …
To that end, we have isolated peptides directly from HLA-A*0201 molecules of human melanoma cells and have determined that naturally process …
Generation of melanoma-specific cytotoxic T lymphocytes for allogeneic immunotherapy.
Nolte A, Scheffold C, Slotty J, Huenefeld C, Schultze JL, Grabbe S, Berdel WE, Kienast J. Nolte A, et al. J Immunother. 2003 May-Jun;26(3):257-69. doi: 10.1097/00002371-200305000-00009. J Immunother. 2003. PMID: 12806279
In contrast, FLWGPRALV(MAGE3(271)-(279)) and VLPDVFIRCV(GnT-V(nt38-67)) induced peptide-specific recognition of T2 target cells only, whereas ITDQVPFSV(G(209-217)), KTWGQYWQV(G9(154)), MLLAVLYCL(T(1-9)), and tumor lysate could not induce specific CTLs. ...
In contrast, FLWGPRALV(MAGE3(271)-(279)) and VLPDVFIRCV(GnT-V(nt38-67)) induced peptide-specific recognition of T2 target cells only, wherea …
Competition among peptides in melanoma vaccines for binding to MHC molecules.
Thompson LW, Garbee CF, Hibbitts S, Brinckerhoff LH, Pierce RA, Chianese-Bullock KA, Deacon DH, Engelhard VH, Slingluff CL Jr. Thompson LW, et al. J Immunother. 2004 Nov-Dec;27(6):425-31. doi: 10.1097/00002371-200411000-00002. J Immunother. 2004. PMID: 15534486
In multiple experiments, two peptides with different affinities for HLA-A*0201 molecules were mixed at various concentrations and pulsed onto HLA-A2 cells, which were then evaluated for susceptibility to lysis by HLA-A*0201-restricted CTLs. CTL recognition of the melanoma peptide …
In multiple experiments, two peptides with different affinities for HLA-A*0201 molecules were mixed at various concentrations and pulsed ont …
Immunization against epitopes in the human melanoma antigen gp100 following patient immunization with synthetic peptides.
Salgaller ML, Marincola FM, Cormier JN, Rosenberg SA. Salgaller ML, et al. Cancer Res. 1996 Oct 15;56(20):4749-57. Cancer Res. 1996. PMID: 8840994 Clinical Trial.
In this study, peripheral blood mononuclear cells (PBMCs) were harvested from HLA-A2+ melanoma patients before and after immunization with G9-209 (ITDQVPFSY), G9-280 (YLEPGPVTA), or G9-154 (KTWGQYWQV) peptides in Incomplete Freund's Adjuvant and were tested for the ability …
In this study, peripheral blood mononuclear cells (PBMCs) were harvested from HLA-A2+ melanoma patients before and after immunization with G …
Improved induction of melanoma-reactive CTL with peptides from the melanoma antigen gp100 modified at HLA-A*0201-binding residues.
Parkhurst MR, Salgaller ML, Southwood S, Robbins PF, Sette A, Rosenberg SA, Kawakami Y. Parkhurst MR, et al. J Immunol. 1996 Sep 15;157(6):2539-48. J Immunol. 1996. PMID: 8805655
Three common gp100 epitopes have been identified that are recognized in the context of HLA-A2 by TIL from different patients: G9154 (KTWGQYWQV), G9209 (ITDQVPFSV), and G9280 (YLEPGPVTA). Upon stimulation with these peptides, melanoma-reactive CTL could be induced in vitro …
Three common gp100 epitopes have been identified that are recognized in the context of HLA-A2 by TIL from different patients: G9154 (KTWG …