In vitro cultivation and partial characterization of Lawsonia intracellularis from a Japanese field case of porcine proliferative enteropathy

J Vet Med Sci. 2006 Jun;68(6):609-13. doi: 10.1292/jvms.68.609.

Abstract

Lawsonia intracellularis isolated from a Japanese field case of porcine proliferative enteropathy (PPE) was cultivated and partially characterized. The bacterial cells isolated from the intestinal mucosa of a pig suffering from the acute form of PPE were used to inoculate rat small intestine cells (IEC-18) and human epithelial cells (HEp-2). Infected foci, which were stained with L. intracellularis-specific rabbit antiserum, were observed in the cell culture at 5 days post inoculation. The DNA sequence of several genes in the Japanese isolate had high similarity with those of the L. intracellularis type strain, suggesting the genetically close relationship of the two strains. This is the first report describing the cultivation and partial characterization of L. intracellularis originated in Japan.

MeSH terms

  • Animals
  • Cell Line
  • DNA, Bacterial
  • Desulfovibrionaceae Infections / epidemiology
  • Desulfovibrionaceae Infections / microbiology
  • Desulfovibrionaceae Infections / veterinary*
  • Enteritis / epidemiology
  • Enteritis / microbiology
  • Enteritis / veterinary*
  • Ileum / microbiology
  • Ileum / pathology
  • Japan / epidemiology
  • Lawsonia Bacteria / isolation & purification*
  • Molecular Sequence Data
  • Swine / microbiology*
  • Swine Diseases / epidemiology
  • Swine Diseases / microbiology*

Substances

  • DNA, Bacterial

Associated data

  • GENBANK/AB218756
  • GENBANK/AB218757
  • GENBANK/AB218758