Effect of yellow capsicum extract on proliferation and differentiation of 3T3-L1 preadipocytes

Nutrition. 2014 Mar;30(3):319-25. doi: 10.1016/j.nut.2013.08.003. Epub 2013 Dec 2.

Abstract

Objectives: To evaluate the effect of effect of Yellow Capsicum extract (YCE) that is rich in capsaicin on the proliferation and differentiation of 3T3-L1 preadipocytes in vitro.

Methods: 3T3 L1 cells that were exposed to differentiation-inducing medium containing high glucose DMEM (Dulbecco's Modified Eagle's Medium) and subsequently were treated with capsaicin and YCE for their effect on adipocyte differentiation, changes in their triglyceride content, leptin secretion, expression of lipoprotein lipase, PPARγ, and CCAAT/enhancer-binding protein alpha (C/EBPα).

Results: Both YCE and capsaicin inhibited proliferation and differentiation 3T3-L1 preadipocytes and suppressed accumulation of intracellular triglyceride in a dose-dependent manner. In addition, a significant decrease in the expression of lipoprotein lipase (LPL), leptin, PPARγ, and C/EBPα was noted in 3T3-L1 preadipocytes when induced to differentiate by YCE and Capsaicin.

Conclusions: The potent inhibitory action of YCE and Capsaicin on the differentiation of 3T3-L1 preadipocyte observed suggests that they (YCE and Capsaicin) have the potential to inhibit obesity that needs to be explored in future studies.

Keywords: 3T3-L1; Differentiation; Obesity; Proliferation; Yellow capsicum extract.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Animals
  • CCAAT-Enhancer-Binding Protein-alpha / genetics
  • CCAAT-Enhancer-Binding Protein-alpha / metabolism
  • Capsaicin / pharmacology
  • Capsicum / chemistry*
  • Cell Cycle / drug effects
  • Cell Differentiation / drug effects*
  • Cell Proliferation / drug effects*
  • Leptin / metabolism
  • Lipoprotein Lipase / genetics
  • Lipoprotein Lipase / metabolism
  • Mice
  • PPAR gamma / genetics
  • PPAR gamma / metabolism
  • Plant Extracts / pharmacology*
  • Triglycerides / metabolism

Substances

  • CCAAT-Enhancer-Binding Protein-alpha
  • Leptin
  • PPAR gamma
  • Plant Extracts
  • Triglycerides
  • Lipoprotein Lipase
  • Capsaicin