Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.
Biotechnol Appl Biochem. 2002.
PMID: 11916451
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Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.
Biotechnol Appl Biochem. 2002 Apr;35(2):91-105. doi: 10.1042/ba20010099.
Biotechnol Appl Biochem. 2002.
PMID: 11916451
Review.
A recent drastic step forward in the use of gene technology to improve recovery processes for recombinant proteins is the introduction of combinatorial protein engineering to generate tailor-made product-specific affinity ligands. ...The examples prese …
A recent drastic step forward in the use of gene technology to improve recovery processes for recombinant proteins is t …
Endolysin-Based Autolytic E. coli System for Facile Recovery of Recombinant Proteins.
J Agric Food Chem. 2021 Mar 17;69(10):3134-3143. doi: 10.1021/acs.jafc.1c00059. Epub 2021 Mar 3.
J Agric Food Chem. 2021.
PMID: 33656890
Recovery of recombinant proteins from the Escherichia coli cytoplasm depends on cell disruption by mechanical, chemical, and/or enzymatic methods, which usually cause incomplete cell breakage or protein denaturation. Controllable autolytic E. …
Recovery of recombinant proteins from the Escherichia coli cytoplasm depends on cell disruption by mechan …
Recombinant production of active Streptococcus pneumoniae CysE in E. coli facilitated by codon optimized BL21(DE3)-RIL and detergent.
Prep Biochem Biotechnol. 2019;49(4):368-374. doi: 10.1080/10826068.2019.1573194. Epub 2019 Feb 8.
Prep Biochem Biotechnol. 2019.
PMID: 30734630
Therefore, it appears to be a very attractive target for structure-function understanding and inhibitor design. This study describes the molecular cloning of cysE from Spn in the pET21c vector and efforts carried out for expression and purification of active recombinant …
Therefore, it appears to be a very attractive target for structure-function understanding and inhibitor design. This study describes …
Maximizing the recovery of the native p28 bacterial peptide with improved activity and maintained solubility and stability in Escherichia coli BL21 (DE3).
J Microbiol Methods. 2022 Sep;200:106560. doi: 10.1016/j.mimet.2022.106560. Epub 2022 Aug 27.
J Microbiol Methods. 2022.
PMID: 36031157
Considering the interesting biological qualities of p28, maximizing its expression appears to be a prominent priority. The optimization of such bioprocesses might be facilitated by utilizing statistical approaches such as Design of Experiment (DoE). In this study, w …
Considering the interesting biological qualities of p28, maximizing its expression appears to be a prominent priority. The optimization of s …
Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination.
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8196-202. doi: 10.1073/pnas.121008898.
Proc Natl Acad Sci U S A. 2001.
PMID: 11459953
Free PMC article.
Review.
Alternative reproductive cycles make use of different strategies to generate different reproductive products. In Escherichia coli, recA and several other rec genes are required for the generation of recombinant genomes during Hfr conjugation. ...We dis …
Alternative reproductive cycles make use of different strategies to generate different reproductive products. In Escherichia …
Simulation of large-scale production of a soluble recombinant protein expressed in Escherichia coli using an intein-mediated purification system.
Appl Biochem Biotechnol. 2005 Aug;126(2):93-118. doi: 10.1385/abab:126:2:093.
Appl Biochem Biotechnol. 2005.
PMID: 16118465
Industrially, an intein-based system could potentially reduce production costs of recombinant proteins by facilitating a highly selective affinity purification using an inexpensive substrate such as chitin. In this study, SuperPro Designer was u …
Industrially, an intein-based system could potentially reduce production costs of recombinant proteins by facilitati …
Preparation of recombinant human thymic stromal lymphopoietin protein.
Prep Biochem Biotechnol. 2017 Oct 21;47(9):934-938. doi: 10.1080/10826068.2017.1365242. Epub 2017 Aug 17.
Prep Biochem Biotechnol. 2017.
PMID: 28816641
In this report, an efficient overexpression system for the production of hTSLP was developed. We constructed expression plasmids of the full-length hTslp gene with or without the signal peptide and transformed the plasmids into Escherichia coli. The design …
In this report, an efficient overexpression system for the production of hTSLP was developed. We constructed expression plasmids of t …
Utilizing chitooligosaccharides from shrimp waste biodegradation via recombinant chitinase A: a promising approach for emulsifying hydrocarbon and bioremediation.
Microb Cell Fact. 2024 May 2;23(1):126. doi: 10.1186/s12934-024-02388-z.
Microb Cell Fact. 2024.
PMID: 38698402
Free PMC article.
This investigation explores two strategies to enhance chitinase productivity, firstly, statistical optimization by the Plackett Burman design approach to evaluating the influence of individual physical and chemical parameters on chitinase production, Followed …
This investigation explores two strategies to enhance chitinase productivity, firstly, statistical optimization by the Plackett Burma …
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