Abstract
Complementary DNA clones encoding the rat PepT1 small-intestinal oligopeptide transporter were isolated from a jejunal library by cross-hybridization with a rabbit PepT1 cDNA probe. The cDNA sequence indicates that rat PepT1 is composed of 710 amino acids and shows 77% and 83% amino acid sequence identity with rabbit and human PepT1, respectively. Northern blot analysis detected rat PepT1 mRNA in the small intestine and kidney. Intestinal PepT1 mRNA levels were highest in 4-day old rats, and then decreased reaching the adult level by day 28 after birth. These results indicate that the expressions of PepT1 gene change markedly during development.
MeSH terms
-
Age Factors
-
Animals
-
Base Sequence
-
Carrier Proteins / genetics*
-
Carrier Proteins / metabolism*
-
DNA, Complementary
-
Gene Expression Regulation, Developmental
-
Humans
-
Intestine, Small / growth & development
-
Intestine, Small / metabolism*
-
Jejunum / growth & development
-
Jejunum / metabolism
-
Kidney / growth & development
-
Kidney / metabolism
-
Molecular Sequence Data
-
Oligopeptides / metabolism*
-
Peptide Transporter 1
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
Rabbits
-
Rats
-
Rats, Sprague-Dawley
-
Sequence Homology, Amino Acid
-
Symporters*
-
Tissue Distribution
Substances
-
Carrier Proteins
-
DNA, Complementary
-
Oligopeptides
-
Peptide Transporter 1
-
RNA, Messenger
-
SLC15A1 protein, human
-
Slc15a1 protein, rat
-
Symporters