Sequence, tissue distribution and developmental changes in rat intestinal oligopeptide transporter

Biochim Biophys Acta. 1996 Feb 7;1305(1-2):34-8. doi: 10.1016/0167-4781(95)00208-1.

Abstract

Complementary DNA clones encoding the rat PepT1 small-intestinal oligopeptide transporter were isolated from a jejunal library by cross-hybridization with a rabbit PepT1 cDNA probe. The cDNA sequence indicates that rat PepT1 is composed of 710 amino acids and shows 77% and 83% amino acid sequence identity with rabbit and human PepT1, respectively. Northern blot analysis detected rat PepT1 mRNA in the small intestine and kidney. Intestinal PepT1 mRNA levels were highest in 4-day old rats, and then decreased reaching the adult level by day 28 after birth. These results indicate that the expressions of PepT1 gene change markedly during development.

Publication types

  • Comparative Study

MeSH terms

  • Age Factors
  • Animals
  • Base Sequence
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • DNA, Complementary
  • Gene Expression Regulation, Developmental
  • Humans
  • Intestine, Small / growth & development
  • Intestine, Small / metabolism*
  • Jejunum / growth & development
  • Jejunum / metabolism
  • Kidney / growth & development
  • Kidney / metabolism
  • Molecular Sequence Data
  • Oligopeptides / metabolism*
  • Peptide Transporter 1
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rabbits
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Homology, Amino Acid
  • Symporters*
  • Tissue Distribution

Substances

  • Carrier Proteins
  • DNA, Complementary
  • Oligopeptides
  • Peptide Transporter 1
  • RNA, Messenger
  • SLC15A1 protein, human
  • Slc15a1 protein, rat
  • Symporters

Associated data

  • GENBANK/D50664