Serum samples from 665 hunter-killed black bears killed in 1989 to 1992 throughout Pennsylvania (USA) were tested for Toxoplasma gondii antibodies by the agglutination test in dilutions of 1:25, 1:50, and 1:500. Toxoplasma gondii antibodies were found in 535 of 665 (80%) bears. Considering the highest dilutions at which antibodies were detected, prevalences were 10% at 1:25, 37% at 1:50 and 33% at 1:500. No significant difference in antibody prevalence was found between males (79%) and females (80%), but a significant difference was found between juvenile (65%) and adult (83%) bears.