Abstract
The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5' end of the intervening sequence joined to a site near the 3' splice site. This lariat structure, which has been characterized for an adenovirus 2 major late transcript, has a branch point, with 2'-5' and 3'-5' phosphodiester bonds emanating from a single adenosine residue. The excised intervening sequence retains the branch site and terminates in a guanosine residue with a 3' hydroxyl group. The phosphate group at the splice junction between the two exons originates from the 3' splice site at the precursor.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviruses, Human / metabolism
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Base Sequence
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Chemical Phenomena
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Chemistry
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Nucleic Acid Conformation
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Nucleic Acid Precursors / analysis
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Nucleic Acid Precursors / metabolism*
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Oligoribonucleotides / metabolism
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Phosphates / metabolism
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RNA / analysis
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RNA / metabolism*
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RNA Precursors
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RNA Splicing*
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RNA, Messenger / analysis
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RNA, Messenger / metabolism*
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RNA, Viral / analysis
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RNA, Viral / metabolism*
Substances
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Nucleic Acid Precursors
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Oligoribonucleotides
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Phosphates
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RNA Precursors
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RNA, Messenger
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RNA, Viral
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RNA
Associated data
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GENBANK/J01917
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GENBANK/J01918
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GENBANK/J01919
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GENBANK/J01920
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GENBANK/J01921
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GENBANK/J01922
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GENBANK/J01923
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GENBANK/J01924
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GENBANK/J01925
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GENBANK/J01926
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GENBANK/J01927
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GENBANK/J01928
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GENBANK/J01929
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GENBANK/J01930
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GENBANK/J01931
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GENBANK/J01932
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GENBANK/J01933
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GENBANK/J01934
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GENBANK/J01935
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GENBANK/J01936
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GENBANK/J01937
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GENBANK/J01938
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GENBANK/J01939
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GENBANK/J01940
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GENBANK/J01941
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GENBANK/J01942
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GENBANK/J01943
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GENBANK/J01944
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GENBANK/J01945
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GENBANK/J01946