Monoclonal radioimmunoassays for hepatitis B surface antigen: demonstration of hepatitis B virus DNA or related sequences in serum and viral epitopes in immune complexes

Proc Natl Acad Sci U S A. 1982 Sep;79(18):5675-9. doi: 10.1073/pnas.79.18.5675.

Abstract

High-affinity monoclonal IgM and IgG antibodies (anti-HBs) to hepatitis B surface antigen (HBsAg) have recently been developed and used by us in the construction of highly sensitive radioimmunoassays for the detection of HBsAg-associated determinants in serum. We now report that selected serum samples demonstrating monoclonal immunoreactive material also contain nucleic acid sequences complementary to hepatitis B virus (HBV) DNA by molecular hybridization analysis. In addition, monoclonal radioimmunoassays can detect viral determinants in HBsAg-anti-HBs immune complexes formed in anti-HBs excess; such determinants are undetectable by commercial radioimmunoassay. These and our previous studies suggest that there are HBV or "HBV-related" agents present in human serum that are detected by monoclonal antibodies but are not identified by conventional polyvalent antibodies.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal*
  • Antigen-Antibody Complex*
  • Blood Donors
  • DNA, Viral / blood
  • DNA, Viral / genetics*
  • Epitopes / analysis*
  • Ethnicity
  • Hepatitis B / immunology
  • Hepatitis B / microbiology*
  • Hepatitis B Surface Antigens / analysis*
  • Hepatitis B virus / genetics*
  • Humans
  • Native Hawaiian or Other Pacific Islander
  • Radioimmunoassay / methods

Substances

  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • DNA, Viral
  • Epitopes
  • Hepatitis B Surface Antigens