Generation of a MIR5004 knockout cell line from human induced pluripotent stem cells by CRISPR/Cas9 gene editing

Maisumu Gulimiheranmu; Shuang Li; Junmei Zhou

doi:10.1016/j.scr.2022.102805

Generation of a MIR5004 knockout cell line from human induced pluripotent stem cells by CRISPR/Cas9 gene editing

Stem Cell Res. 2022 Jul:62:102805. doi: 10.1016/j.scr.2022.102805. Epub 2022 May 4.

Authors

Maisumu Gulimiheranmu¹, Shuang Li¹, Junmei Zhou²

Affiliations

¹ Department of Central Laboratory, Shanghai Children's Hospital, School of medicine, Shanghai Jiao Tong University, 355 Luding Road, Shanghai 200062, China.
² Department of Central Laboratory, Shanghai Children's Hospital, School of medicine, Shanghai Jiao Tong University, 355 Luding Road, Shanghai 200062, China. Electronic address: zhoujm@shchildren.com.cn.

PMID: 35576811
DOI: 10.1016/j.scr.2022.102805

Abstract

MIR5004 is located in the intronic region of SYNGAP1, a genetic risk factor for Autism Spectrum Disorders (ASD), and co-expressed with SYNGAP1 in brain tissue, which indicates that MIR5004 may play an important role in ASD pathogenesis through the regulation of SYNGAP1. Here, we generated a MIR5004 knockout human induced pluripotent stem cell (iPSC) line SHCDCLi001-B. SHCDCLi001-B shows expression of pluripotent markers, three lineage differentiation capacity, normal morphology and karyotypes, the same DNA origin with wild type iPSC (iPSC-WT) and no off-target effects, making it as a valuable tool for studying the interplay between MIR5004 and SYNGAP1 in ASD pathogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Cell Differentiation / genetics
Cell Line
Gene Editing*
Humans
Induced Pluripotent Stem Cells* / metabolism