A molecular Rosetta Stone to decipher the impact of chromatin features on the repair of Cas9-mediated DNA double-strand breaks

Pierre Caron; Enrico Pobega; Sophie E Polo

doi:10.1016/j.molcel.2021.04.024

A molecular Rosetta Stone to decipher the impact of chromatin features on the repair of Cas9-mediated DNA double-strand breaks

Mol Cell. 2021 May 20;81(10):2059-2060. doi: 10.1016/j.molcel.2021.04.024.

Authors

Pierre Caron¹, Enrico Pobega¹, Sophie E Polo²

Affiliations

¹ Laboratory of Epigenome Integrity, Epigenetics & Cell Fate Centre, UMR 7216 CNRS - University of Paris, 35 rue Hélène Brion, 75205 Paris Cedex 13, France.
² Laboratory of Epigenome Integrity, Epigenetics & Cell Fate Centre, UMR 7216 CNRS - University of Paris, 35 rue Hélène Brion, 75205 Paris Cedex 13, France. Electronic address: sophie.polo@univ-paris-diderot.fr.

PMID: 34019786
DOI: 10.1016/j.molcel.2021.04.024

Abstract

Using a barcoded reporter introduced within a thousand different chromatin locations in human cells, (Schep et al., 2021) characterize repair outcomes of Cas9-induced DNA double-strand breaks (DSBs) and the relative use of DSB repair pathways depending on the local chromatin context.

Publication types

Comment

MeSH terms

CRISPR-Cas Systems
Chromatin* / genetics
DNA
DNA Breaks, Double-Stranded*
DNA Repair
Humans

Substances

Chromatin
DNA