In the present study, a simple, cheaply and environmental friendly method was evaluated for the synthesis of silver nanoparticle via Cupressus sempervirens L. (CSPE) pollen extract as reducing and stabilizing agent. Various parameters such as volume of CSPE, temperature and reaction time on AgNPs formation were investigated spectrophotometrically to optimize reaction conditions. The electrochemical behavior of the biosynthesized AgNPs were investigated by cyclic voltammetry and square wave voltammetry techniques. An electrosensor based on AgNPs modified glassy carbon electrode were constructed and tested on electro reduction of hydrogen peroxide in phosphate buffer medium. The prepared electrosensor could detect the H2O2 in the range of 5.0 μM - 2.5 mM with a detection limit of 0.23 μM. In addition, the antioxidant activity of biosynthesized AgNPs were evaluated against DPPH free radical. Results obtained from the antioxidant study suggested that CSPE mediated AgNPs exhibit a good antioxidant effect.
Keywords: Electrosensor; Green synthesis; Hydrogen peroxide; Nanoparticles; Pollen; Square wave voltammetry.
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