Pedobacter puniceum sp. nov. Isolated from Sludge

Strain HX-22-1^T was isolated from the sludge collected from the outlet of the biochemical treatment facility of an agricultural chemical factory in Nanjing city, Jiangsu province, PR China. Strain HX-22-1^T is Gram-staining-negative, rod-shaped, non-spore-forming and non-flagellated. The 16S rRNA gene-based phylogenetic analysis indicates that the strain HX-22-1^T belongs to the genus Pedobacter, closely related to Pedobacter glucosidilyticus KCTC 22438^T (98.63% similarity). The genomic DNA G+C content of the strain was 34.4 mol%. Strain HX-22-1^T was able to grow at 16-37 °C (optimum at 30 °C), at pH 6.0-8.0 (optimum at pH 7.0), and with 0-1% (w/v) NaCl (optimum at 0). Predominant fatty acid constituents were iso-C_15:0 and summed feature 3 (iso-C_16:1ω7c and/or C_16:1ω6c). The predominant respiratory ubiquinone was MK-7. The polar lipid profile is composed of phosphatidylethanolamine (PE), aminophospholipid (APL), aminolipid (AL), and phospholipids (PL). The ANI and dDDH values obtained between the genomes of HX-22-1^T and P. glucosidilyticus KCTC 22438^T were 89.6 and 38.8%, respectively. On the basis of data from phenotypic, chemotaxonomic, and genotypic analysis, strain HX-22-1^T represents a novel species of the genus Pedobacter, for which the name Pedobacter puniceum sp. nov. is proposed. The type strain is HX-22-1^T (= KCTC 72655^T = CCTCC AB 2019348^T).