Inducing mesenchymal stem cell attachment on non-cell adhesive hydrogels through click chemistry

Chem Commun (Camb). 2020 Jul 9;56(55):7661-7664. doi: 10.1039/d0cc03403g.

Abstract

We introduce an innovative approach to adhere mesenchymal stem cells (MSCs) to a hydrogel scaffold by nucleating adhesion through strain-promoted click chemistry. This method yields a significant increase in cell viability compared to non-functionalized and RGD peptide functionalized hydrogels, providing a promising alternative to traditional biomaterials cell attachment approaches.

MeSH terms

  • Alkynes / chemistry
  • Azides / chemistry
  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / pharmacology
  • Cell Adhesion* / drug effects
  • Cell Survival / drug effects
  • Click Chemistry*
  • Cycloaddition Reaction
  • Humans
  • Hydrogels / chemistry*
  • Hydrogels / pharmacology
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Microscopy, Confocal
  • Oligopeptides / chemistry
  • Polysaccharides, Bacterial / chemistry

Substances

  • Alkynes
  • Azides
  • Biocompatible Materials
  • Hydrogels
  • Oligopeptides
  • Polysaccharides, Bacterial
  • gellan gum
  • arginyl-glycyl-aspartic acid