Abstract
We introduce an innovative approach to adhere mesenchymal stem cells (MSCs) to a hydrogel scaffold by nucleating adhesion through strain-promoted click chemistry. This method yields a significant increase in cell viability compared to non-functionalized and RGD peptide functionalized hydrogels, providing a promising alternative to traditional biomaterials cell attachment approaches.
MeSH terms
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Alkynes / chemistry
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Azides / chemistry
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Biocompatible Materials / chemistry*
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Biocompatible Materials / pharmacology
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Cell Adhesion* / drug effects
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Cell Survival / drug effects
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Click Chemistry*
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Cycloaddition Reaction
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Humans
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Hydrogels / chemistry*
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Hydrogels / pharmacology
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Mesenchymal Stem Cells / cytology
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Mesenchymal Stem Cells / metabolism
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Microscopy, Confocal
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Oligopeptides / chemistry
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Polysaccharides, Bacterial / chemistry
Substances
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Alkynes
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Azides
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Biocompatible Materials
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Hydrogels
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Oligopeptides
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Polysaccharides, Bacterial
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gellan gum
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arginyl-glycyl-aspartic acid