Multistate proteinous biomemory device based on redox controllable hapten cross-linker

Mater Sci Eng C Mater Biol Appl. 2017 Oct 1:79:336-342. doi: 10.1016/j.msec.2017.05.026. Epub 2017 May 5.

Abstract

A multistate biomemory device consisting of cytochrome c (Cyt-c) photosensitively cross-linked by MACys-Ru(bipyr)2-MACys hapten molecules, which have memory effect through a charge transfer mechanism, has been developed. In this study, it has suggested a highly resolute surface-confined switch composed a signal-enhanced electro-active protein (Cyt-c) co-polymerized on the gold substrates that can be controlled by redox property through Ruthenium based cysteine monomer hapten, MACys-Ru(bipyr)2-MACys as an ANADOLUCA photosensitive cross-linker. The photosensitive cross-linking of the Cyt-c protein on the gold surface topography has been determined by the scanning electron microscopy (SEM). Two state memory functions, writing and erasing of the developed biomemory device, have been investigated by the chronoamperometry (CA) and open-circuit potential amperometry (OCPA). The polymeric proteinous memory device, p(MACys-Ru(bipyr)2-MACys-co-Cyt-c) layer, on the gold electrode is stable and repeatable up to with 104 times continuous cycle.

Keywords: Multistate biomemory device; Nanobiochip; Open-circuit potential amperometry; Polymeric proteinous memory layer; Ruthenium based amino acid monomer hapten.

MeSH terms

  • Cross-Linking Reagents
  • Cytochromes c
  • Electrodes
  • Equipment Design
  • Gold
  • Haptens / chemistry*
  • Oxidation-Reduction

Substances

  • Cross-Linking Reagents
  • Haptens
  • Gold
  • Cytochromes c