Hedgehog Signaling Regulates the Ciliary Transport of Odorant Receptors in Drosophila

Gonzalo M Sanchez; Liza Alkhori; Eduardo Hatano; Sebastian W Schultz; Anujaianthi Kuzhandaivel; Shadi Jafari; Björn Granseth; Mattias Alenius

doi:10.1016/j.celrep.2015.12.059

Hedgehog Signaling Regulates the Ciliary Transport of Odorant Receptors in Drosophila

Cell Rep. 2016 Jan 26;14(3):464-470. doi: 10.1016/j.celrep.2015.12.059. Epub 2016 Jan 7.

Authors

Gonzalo M Sanchez¹, Liza Alkhori¹, Eduardo Hatano¹, Sebastian W Schultz¹, Anujaianthi Kuzhandaivel¹, Shadi Jafari¹, Björn Granseth¹, Mattias Alenius²

Affiliations

¹ Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden.
² Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden. Electronic address: mattias.alenius@liu.se.

PMID: 26774485
DOI: 10.1016/j.celrep.2015.12.059

Abstract

Hedgehog (Hh) signaling is a key regulatory pathway during development and also has a functional role in mature neurons. Here, we show that Hh signaling regulates the odor response in adult Drosophila olfactory sensory neurons (OSNs). We demonstrate that this is achieved by regulating odorant receptor (OR) transport to and within the primary cilium in OSN neurons. Regulation relies on ciliary localization of the Hh signal transducer Smoothened (Smo). We further demonstrate that the Hh- and Smo-dependent regulation of the kinesin-like protein Cos2 acts in parallel to the intraflagellar transport system (IFT) to localize ORs within the cilium compartment. These findings expand our knowledge of Hh signaling to encompass chemosensory modulation and receptor trafficking.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Behavior, Animal
Calcium / metabolism
Cilia / metabolism*
Drosophila / metabolism*
Drosophila Proteins / antagonists & inhibitors
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Kinesins / metabolism
Mutagenesis
Odorants
RNA Interference
RNA, Small Interfering / metabolism
Receptors, Cell Surface / antagonists & inhibitors
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Receptors, G-Protein-Coupled / antagonists & inhibitors
Receptors, G-Protein-Coupled / genetics
Receptors, G-Protein-Coupled / metabolism
Receptors, Odorant / genetics
Receptors, Odorant / metabolism*
Signal Transduction
Smoothened Receptor

Substances

Drosophila Proteins
RNA, Small Interfering
Receptors, Cell Surface
Receptors, G-Protein-Coupled
Receptors, Odorant
Smoothened Receptor
cos protein, Drosophila
ptc protein, Drosophila
smo protein, Drosophila
Kinesins
Calcium