LncRNA MALAT1 functions as a competing endogenous RNA to regulate ZEB2 expression by sponging miR-200s in clear cell kidney carcinoma

Oncotarget. 2015 Nov 10;6(35):38005-15. doi: 10.18632/oncotarget.5357.

Abstract

Long non-coding RNA (lncRNAs) play a critical role in the development of cancers. LncRNA metastasis-associated lung adenocarcinoma transcript 1(MALAT1) has recently been identified to be involved in tumorigenesis of several cancers such as lung cancer, bladder cancer and so on. Here, we found that MALAT1 exist a higher fold change (Tumor/Normal) in clear cell kidney carcinoma (KIRC) from The Cancer Genome Atlas (TCGA) Data Portal and a negative correlation with miR-200s family. We further demonstrated MALAT1 promote KIRC proliferation and metastasis through sponging miR-200s in vitro and in vivo. In addition, miR-200c can partly reverse the MALAT1's stimulation on proliferation and metastasis in KIRC. In summary we unveil a branch of the MALAT1/miR-200s/ZEB2 pathway that regulates the progression of KIRC. The inhibition of MALAT1 expression may be a promising strategy for KIRC therapy.

Keywords: KIRC; MALAT1; ZEB2; lncRNA; miR-200s.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Blotting, Western
  • Carcinoma, Renal Cell / genetics*
  • Carcinoma, Renal Cell / pathology
  • Cell Cycle
  • Cell Movement
  • Cell Proliferation
  • Cell Transformation, Neoplastic
  • Disease Progression
  • Gene Expression Regulation, Neoplastic*
  • Homeodomain Proteins / genetics*
  • Humans
  • Immunoprecipitation
  • Kidney Neoplasms / genetics
  • Kidney Neoplasms / pathology
  • Lung Neoplasms / genetics*
  • Lung Neoplasms / secondary
  • Mice
  • MicroRNAs / genetics*
  • RNA / genetics*
  • RNA, Long Noncoding / genetics*
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Repressor Proteins / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • Xenograft Model Antitumor Assays
  • Zinc Finger E-box Binding Homeobox 2

Substances

  • Homeodomain Proteins
  • MALAT1 long non-coding RNA, human
  • MIRN200 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • RNA, Messenger
  • Repressor Proteins
  • ZEB2 protein, human
  • Zinc Finger E-box Binding Homeobox 2
  • RNA