Uncleavable Nup98-Nup96 is functional in the fission yeast Schizosaccharomyces pombe

Haruhiko Asakawa; Chie Mori; Chizuru Ohtsuki; Masaaki Iwamoto; Yasushi Hiraoka; Tokuko Haraguchi

doi:10.1016/j.fob.2015.06.004

Uncleavable Nup98-Nup96 is functional in the fission yeast Schizosaccharomyces pombe

FEBS Open Bio. 2015 Jun 12:5:508-14. doi: 10.1016/j.fob.2015.06.004. eCollection 2015.

Authors

Haruhiko Asakawa¹, Chie Mori², Chizuru Ohtsuki¹, Masaaki Iwamoto², Yasushi Hiraoka³, Tokuko Haraguchi³

Affiliations

¹ Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita 565-0871, Japan.
² Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan.
³ Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita 565-0871, Japan ; Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan.

Abstract

Essential nucleoporins Nup98 and Nup96 are coded by a single open reading frame, and produced by autopeptidase cleavage. The autocleavage site of Nup98-Nup96 is highly conserved in a wide range of organisms. To understand the importance of autocleavage, we examined a mutant that produces the Nup98-Nup96 joint molecule as a sole protein product of the nup189 (+) gene in the fission yeast Schizosaccharomyces pombe. Cells expressing only the joint molecule were found to be viable. This result indicates that autocleavage of Nup98-Nup96 is dispensable for cell growth, at least under normal culture conditions in S. pombe.

Keywords: Alternative splicing; FG nup; Fission yeast; GLFG repeat, Gly-Leu-Phe-Gly repeat; NE, nuclear envelope; NPC, nuclear pore complex; Nuclear pore complex; Nucleoporins; Nup107-160 complex.