Abstract
Optical spectroscopic methods do not routinely provide information on higher order hierarchical structure (tertiary/quaternary) of biological macromolecules and assemblies. This necessitates the use of time-consuming and material intensive techniques, such as protein crystallography, NMR, and electron microscopy. Here we demonstrate a spectroscopic phenomenon, superchiral polarimetry, which can rapidly characterize ligand-induced changes in protein higher order (tertiary/quaternary) structure at the picogram level, which is undetectable using conventional CD spectroscopy. This is achieved by utilizing the enhanced sensitivity of superchiral evanescent fields to mesoscale chiral structure.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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3-Phosphoshikimate 1-Carboxyvinyltransferase / chemistry
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Buffers
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Circular Dichroism
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Dickeya chrysanthemi / enzymology
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Escherichia coli / enzymology
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Ligands
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Macromolecular Substances
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Microscopy, Electron, Scanning
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Nanostructures / chemistry*
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Phosphotransferases (Alcohol Group Acceptor) / chemistry
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Protein Structure, Quaternary
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Protein Structure, Secondary
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Protein Structure, Tertiary
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Proteins / chemistry*
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Spectrophotometry / methods*
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Stereoisomerism
Substances
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Buffers
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Ligands
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Macromolecular Substances
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Proteins
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3-Phosphoshikimate 1-Carboxyvinyltransferase
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Phosphotransferases (Alcohol Group Acceptor)
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shikimate kinase