Reversibly Triggered Protein-Ligand Assemblies in Giant Vesicles

Ruud J R W Peters; Marlies Nijemeisland; Jan C M van Hest

doi:10.1002/anie.201502920

Reversibly Triggered Protein-Ligand Assemblies in Giant Vesicles

Angew Chem Int Ed Engl. 2015 Aug 10;54(33):9614-7. doi: 10.1002/anie.201502920. Epub 2015 Jun 18.

Authors

Ruud J R W Peters¹, Marlies Nijemeisland¹, Jan C M van Hest²

Affiliations

¹ Bio-organic chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands).
² Bio-organic chemistry, Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands). j.vanhest@science.ru.nl.

PMID: 26089190
DOI: 10.1002/anie.201502920

Abstract

External small-molecule triggers were used to reversibly control dynamic protein-ligand interactions in giant vesicles. An alcohol dehydrogenase was employed to increase or decrease the interior pH upon conversion of two different small-molecule substrates, thereby modulating the pH-sensitive interaction between a Ni-NTA ligand on the vesicle membrane and an oligohistidine-tagged protein in the lumen. By alternating the small-molecule substrates the interaction could be reversed.

Keywords: cell mimic; liposomes; protein-ligand interactions; vesicles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Dehydrogenase / metabolism
Artificial Cells / metabolism*
Hydrogen-Ion Concentration
Ligands
Liposomes / metabolism*
Luminescent Proteins / metabolism*
Microscopy, Confocal
Microscopy, Fluorescence
Protein Binding
Proteins / metabolism*

Substances

Ligands
Liposomes
Luminescent Proteins
Proteins
Alcohol Dehydrogenase