The complete mitochondrial sequence of Pseudogyrinocheilus prochilus, an endemic species to China and mainly distributed in upper Yangtze River and its tributaries, was obtained by PCR. It was 16,595 bp in length and comprised 37 mitochondrial genes (22 transfer RNA genes, 13 protein-coding genes, 2 ribosomal RNA genes) and 2 non-coding regions. The overall base composition of H-strand was 31.40% A, 26.78% C, 16.18% G, and 25.64% T. Most of the genes are encoded on heavy strand, except for eight tRNA and ND6 genes. The composition and order of mitochondrial gene have no unique characteristics compared to most of other fishes.
Keywords: Complete mitochondrial genome; Labeoninae; Pseudogyrinocheilus prochilus.