Abstract
Multivalent synthetic vaccines were obtained by solid-phase synthesis of tumor-associated MUC1 glycopeptide antigens and their coupling to a Pam3 Cys lipopeptide through click reactions. These vaccines elicited immune responses in mice without the use of any external adjuvant. The vaccine containing four copies of a MUC1 sialyl-TN antigen showed a significant cluster effect. It induced in mice prevailing IgG2a antibodies, which bind to MCF-7 breast tumor cells and initiate the killing of these tumor cells by activation of the complement-dependent cytotoxicity complex.
Keywords:
antigens; antitumor agents; cytotoxicity; multivalent glycopeptides; vaccines.
Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Antibodies / immunology
-
Antibodies / pharmacology
-
Apoptosis / drug effects
-
Cancer Vaccines / chemical synthesis
-
Cancer Vaccines / chemistry
-
Cancer Vaccines / immunology*
-
Click Chemistry
-
Epitopes, T-Lymphocyte / chemistry
-
Epitopes, T-Lymphocyte / immunology
-
Glycopeptides / chemical synthesis
-
Glycopeptides / chemistry*
-
Humans
-
Lipoproteins / chemical synthesis
-
Lipoproteins / chemistry*
-
MCF-7 Cells
-
Mice
-
Mice, Inbred BALB C
-
Mucin-1 / chemistry*
-
Mucin-1 / immunology
-
Mucin-1 / metabolism
-
Rabbits
Substances
-
Antibodies
-
Cancer Vaccines
-
Epitopes, T-Lymphocyte
-
Glycopeptides
-
Lipoproteins
-
Mucin-1
-
N-palmitoyl-S-(2,3-bis(palmitoyloxy)propyl)cysteinyl-seryl-lysyl-lysyl-lysyl-lysine