Antitags: SERS-encoded nanoparticle assemblies that enable single-spot multiplex protein detection

Adv Mater. 2014 Mar 26;26(12):1941-6. doi: 10.1002/adma.201304107. Epub 2013 Dec 16.

Abstract

Simultaneous detection of multiple proteins on a single spot can be efficiently achieved by using multiplexed surface-enhanced Raman spectroscopy (SERS)-encoded nanoparticle 'antitags' consisting of poly(ethylene glycol) (PEG)-protected silver dimers (and higher aggregates) and antibody-tagging entities. The effective SERS-based multivariate deconvolution approach guarantees an accurate and successful distinguishable identification of single and multiple proteins in complex samples. Their potential application in multiplexed SERS bioimaging technology can be easily envisaged.

Keywords: biosensors; multiplex; nanoparticles; silver; surface enhanced raman spectroscopy (SERS).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies / chemistry
  • Biphenyl Compounds / chemistry
  • C-Reactive Protein / chemistry
  • Humans
  • Metal Nanoparticles / chemistry*
  • Polyethylene Glycols / chemistry*
  • Protein Multimerization
  • Proteins / chemistry*
  • Silver Compounds / chemistry*
  • Spectrum Analysis, Raman / methods*
  • Sulfhydryl Compounds / chemistry
  • Thrombin / chemistry

Substances

  • 1,4-benzenedithiol
  • Antibodies
  • Biphenyl Compounds
  • Proteins
  • Silver Compounds
  • Sulfhydryl Compounds
  • Polyethylene Glycols
  • C-Reactive Protein
  • Thrombin