Abstract
The 3' end of rsbU, encoding the positive regulator of the stress factor sigma B, was identified as a hot spot for spontaneous IS256 insertion in Staphylococcus aureus SA137/93G. Interestingly, subinhibitory concentrations of chloramphenicol in combination with heat stress, as well as linezolid and spectinomycin at physiological temperatures, selected for such rsbU::IS256 insertion mutants. In consequence of the inactivation of rsbU, the IS256 transposition frequency was increased 4-fold in S. aureus HG001.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetamides / pharmacology
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Anti-Bacterial Agents / pharmacology*
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Chloramphenicol / pharmacology
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DNA Transposable Elements / genetics*
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Gene Expression Regulation, Bacterial / drug effects
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Gene Expression Regulation, Bacterial / genetics
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Linezolid
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Mutation
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Oxazolidinones / pharmacology
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Spectinomycin / pharmacology
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / genetics*
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Temperature
Substances
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Acetamides
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Anti-Bacterial Agents
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DNA Transposable Elements
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Oxazolidinones
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Chloramphenicol
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Spectinomycin
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Linezolid