Single embryo-coupled gate field effect transistor for elective single embryo transfer

Anal Chem. 2013 Jul 16;85(14):6633-8. doi: 10.1021/ac4001018. Epub 2013 Jun 28.

Abstract

In this study, we have proposed and demonstrated experimentally a novel monitoring device of single mouse embryo activity after in vitro fertilization (IVF) using a semiconductor-based field effect transistor (FET). The FET biosensor realized to detect it noninvasively, quantitatively, and continuously by change of hydrogen ions with positive charges, which were induced by dissolved carbon dioxide due to cellular respiration activity during cleavage. The electrical signal of FET biosensor should become an effective indication to evaluate objectively single embryo activity as its morphology is observed subjectively after IVF. The platform based on the FET biosensor will contribute to promote elective single embryo transfer (eSET) in human assisted reproductive technology (ART).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Embryo Culture Techniques / instrumentation
  • Embryo Culture Techniques / methods
  • Female
  • Male
  • Mice
  • Mice, Inbred ICR
  • Pregnancy
  • Single Embryo Transfer / instrumentation
  • Single Embryo Transfer / methods*
  • Transistors, Electronic*